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EMAGE:4521

Ltbp1 latent transforming growth factor beta binding protein 1 ( MGI:109151)
TS15 (9.5 dpc)
in situ reporter

Data Images
EMAGE:4521 EMAGE:4521 EMAGE:4521
Fig3A, Todorovic V; Frendewey D; Gutstein DE; Chen Y; Freyer L; Finnegan E; Liu F; Murphy A; Valenzuela D; Yancopoulos G; Rifkin DB. 2007 [doi:10.1242/dev.008599] Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig3B, Todorovic V; Frendewey D; Gutstein DE; Chen Y; Freyer L; Finnegan E; Liu F; Murphy A; Valenzuela D; Yancopoulos G; Rifkin DB. 2007 [doi:10.1242/dev.008599] Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright. Fig3C, Todorovic V; Frendewey D; Gutstein DE; Chen Y; Freyer L; Finnegan E; Liu F; Murphy A; Valenzuela D; Yancopoulos G; Rifkin DB. 2007 [doi:10.1242/dev.008599] Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: In A and B the red arrow marks the thyroid primordium. In B and C: myocardial cells underlying outflow tract (OFT; B) and atrio-ventricular (AV; C) endocardial cushions express Ltbp1L (black arrows). This specimen is either a heterozygote for "allele A" (Ltbp1<tm1Veto>/Ltbp1<+>) or "allele B" (Ltbp1<tm2Veto>/Ltbp1<+>). The authors state that A and B lines, with or without the neoR cassette, showed the same phenotype; hence, both lines were used interchangeably with no differences observed.
Expression Pattern Description
Spatial Annotation:
EMAGE:4521Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4521_wholemount_moderate_3D_1.wlz
4521_wholemount_weak_3D_1.wlz
4521_wholemount_notDetected_3D_1.wlz
4521_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4521_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
outflow tract
detected detected
single cellExpressed in myocardial cells underlying the outflow tract.
atrioventricular canal
detected detected
single cellExpressed in myocardial cells underlying the atrio-ventricular endocardial cushions.
endocardial cushion tissue
detected detected
single cellExpressed in myocardial cells underlying the atrio-ventricular endocardial cushions.
thyroid primordium
detected detected
Editors note: although the authors do not specifically state that expression is found in the thyroid primordium, the red arrow in Figs 3A and 3B points to the thyroid primordium, and this region has obvious staining.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Ltbp1, latent transforming growth factor beta binding protein 1 ( MGI:109151)
Notes:Ltbp1 (Ltbp1L ) expression in this study by Todorovic et al., 2007 [PMID:17804598] was assayed using one of two Ltbp1L deletion alleles, designated as A and B (see Fig. 1B therein). A allele = a 666 bp deletion including 457 bp of the 3' portion of exon 1 and 209 bp of the downstream intron. B allele = a 7.8 kb deletion from the 165th codon of the Ltbp1L coding sequence in exon 2 through the remainder of exon 2 and into the downstream intron. The deleted sequences were replaced with an expression cassette comprising a beta-galactosidase coding sequence fused in-frame with the upstream coding sequence of Ltbp1L, followed by a polyadenylation signal and an antibiotic selection cassette flanked by loxP sites. The targeting of embryonic stem (ES) cells and the germline transmission were confirmed by a quantitative reverse transcription PCR assay that scored for the loss of one of the native Ltbp1L alleles (data not shown). The neoR cassette was removed by crossing line A and B mice with mice expressing Cre recombinase in the germ cell lineage. A and B lines, with or without the neoR cassette, showed the same phenotype; hence, both lines were used interchangeably with no differences observed.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:involves: 129 * C57BL/6
Age:9.5 dpc
Theiler Stage:TS15
Mutations:Ltbp1tm1Veto/Ltbp1+
Preparation:wholemount
Procedures
General Information
Authors:Todorovic V; Frendewey D; Gutstein DE; Chen Y; Freyer L; Finnegan E; Liu F; Murphy A; Valenzuela D; Yancopoulos G; Rifkin DB. 2007 [PMID:17804598] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.008599] [ PMID:17804598] Todorovic V, Frendewey D, Gutstein DE, Chen Y, Freyer L, Finnegan E, Liu F, Murphy A, Valenzuela D, Yancopoulos G, Rifkin DB 2007 Long form of latent TGF-beta binding protein 1 (Ltbp1L) is essential for cardiac outflow tract septation and remodeling. Development (134):3723-32
Links:MGI:3765966 allele description
 MGI:3765968 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE