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EMAGE:4524

Macf1 microtubule-actin crosslinking factor 1 ( MGI:108559)
TS14 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:4524
Fig3A. Copyright: This image is from 3.0.CO;2-O [doi:10.1002/1097-0177(2000)9999:9999::AID-DVDY1041>3.0.CO;2-O] Bernier G; Pool M; Kilcup M; Alfoldi J; De Repentigny Y; Kothary R, "Acf7 (MACF) is an actin and microtubule linker protein whose expression predominates in neural, muscle, and lung development." Dev Dyn 2000 2191(2):216-25, and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: specific hybridization is observed in the internal portion of the somites (thin arrows), the embryonic heart, and in the closure of the cephalic neural folds (large arrow).
Expression Pattern Description
Spatial Annotation:
EMAGE:4524Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4524_wholemount_moderate_3D_1.wlz
4524_wholemount_weak_3D_1.wlz
4524_wholemount_possible_3D_1.wlz
4524_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4524_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalexpressed in "internal portion of the somites"
heart
detected detected
rostral neuropore
detected detected
Expressed in "closure of the cephalic neural folds"
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Macf1 probeA
Entity Detected:Macf1, microtubule-actin crosslinking factor 1 ( MGI:108559)
Notes:The Macf1 (Acf7) probe template used in this study by Bernier et al., 2000 [PMID:11002341] is described as "a DNA fragment containing Acf7 cDNA sequence common to the different isoforms".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:8.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Bernier G; Pool M; Kilcup M; Alfoldi J; De Repentigny Y; Kothary R, 2000 [PMID:11002341] . Indexed and patially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/1097-0177(2000)9999:9999<::AID-DVDY1041>3.0.CO;2-O] [ PMID:11002341] Bernier G, Pool M, Kilcup M, Alfoldi J, De Repentigny Y, Kothary R 2000 Acf7 (MACF) is an actin and microtubule linker protein whose expression predominates in neural, muscle, and lung development. Dev Dyn (219):216-25
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE