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EMAGE:4525

Maf avian musculoaponeurotic fibrosarcoma (v-maf) AS42 oncogene homolog ( MGI:96909)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4525
Fig 1A-10.5 Kawauchi S; Takahashi S; Nakajima O; Ogino H; Morita M; Nishizawa M; Yasuda K; Yamamoto M. 1999. Regulation of lens fiber cell differentiation by transcription factor c-Maf. J Biol Chem. 274(27):19254-60. [PMID:10383433]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4525Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4525_wholemount_possible_3D_1.wlz
4525_wholemount_notDetected_3D_1.wlz
4525_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4525_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
lens pit
detected detected
expression in the developing lens
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Maf probeA
Entity Detected:Maf, avian musculoaponeurotic fibrosarcoma (v-maf) AS42 oncogene homolog ( MGI:96909)
Notes:The Maf (c-Maf) probe used in this study by Kawauchi et al., 1999 [PMID:10383433] was one of two possible probes (or a mixture of the two): The authors state "Two independent RNA probes were prepared from the 5' and 3' end regions of the c-Maf cDNA.", and "two ORF regions (-5 to 402 and 753 to 1127) of the c-maf gene were subcloned, and sense and antisense probes were synthesized using the T3 and T7 RNA polymerases".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kawauchi S; Takahashi S; Nakajima O; Ogino H; Morita M; Nishizawa M; Yasuda K; Yamamoto M. 1999 [PMID:10383433] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1074/jbc.274.27.19254] [ PMID:10383433] Kawauchi S, Takahashi S, Nakajima O, Ogino H, Morita M, Nishizawa M, Yasuda K, Yamamoto M 1999 Regulation of lens fiber cell differentiation by transcription factor c-Maf. J Biol Chem (274):19254-60
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE