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EMAGE:453

Twist1 twist homolog 1 (Drosophila) ( MGI:98872)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:453
Fig 6B of Solloway & Robertson, 1999 [PMID:10079236] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:453Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
453_wholemount_strong_3D_1.wlz
453_wholemount_moderate_3D_1.wlz
453_wholemount_weak_3D_1.wlz
453_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:453_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme
detected detected
mesenchyme derived from somatopleure
detected detected
trunk paraxial mesenchyme
detected detected
extraembryonic component
detected detected
regionalExpression is in the mesenchyme of the allantois.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1276127
Entity Detected:Twist1, twist homolog 1 (Drosophila) ( MGI:98872)
Notes:The Twist1 (M-twist) probe used in this study by Solloway & Robertson, 1999 [PMID:10079236] is indicated as that previously described by Fuchtbauer, 1995 [PMID:8573722] ie. "in vitro transcribed riboprobes of both the 5' and 3' EcoRI-XbaI (Wolf et al., 1991 [PMID:1840517] ) fragment of the M-twist cDNA were used and gave identical results with no background." Not indicated which fragment was used here.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Solloway & Robertson, 1999 [PMID:10079236] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10079236] Solloway MJ, Robertson EJ 1999 Early embryonic lethality in Bmp5;Bmp7 double mutant mice suggests functional redundancy within the 60A subgroup. Development (126):1753-68
 [ doi:10.1016/0012-1606(91)90086-I] [ PMID:1840517] Wolf C, Thisse C, Stoetzel C, Thisse B, Gerlinger P, Perrin-Schmitt F 1991 The M-twist gene of Mus is expressed in subsets of mesodermal cells and is closely related to the Xenopus X-twi and the Drosophila twist genes. Dev Biol (143):363-73
 [ doi:10.1002/aja.1002040309] [ PMID:8573722] Fuchtbauer EM 1995 Expression of M-twist during postimplantation development of the mouse. Dev Dyn (204):316-22
Links:MGI:1333106 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE