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EMAGE:4534

Map2k5 mitogen-activated protein kinase kinase 5 ( MGI:1346345)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4534 EMAGE:4534
Fig3-E10.5_MKK5. [doi:10.1186/1471-213X-3-11] [PMID:14675480] Copyright: This image is from Yan L; Carr J; Ashby PR; Murry-Tait V; Thompson C; Arthur JS, "Knockout of ERK5 causes multiple defects in placental and embryonic development. " BMC Dev Biol 2003 3:11, an open-access article distributed under the terms of theCreative Commons Attribution License Fig3-E10.5_control. [doi:10.1186/1471-213X-3-11] [PMID:14675480] Copyright: This image is from Yan L; Carr J; Ashby PR; Murry-Tait V; Thompson C; Arthur JS, "Knockout of ERK5 causes multiple defects in placental and embryonic development. " BMC Dev Biol 2003 3:11, an open-access article distributed under the terms of the Creative Commons Attribution License

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Whole mount in situ hybridisation was carried out on wild-type embryos using antisense RNA probe against MKK5 (first image) or with no RNA probe (second image). Editors note: the forebrain region is missing from the embryo stained for MKK5 expression.
Expression Pattern Description
Spatial Annotation:
EMAGE:4534Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4534_wholemount_moderate_3D_1.wlz
4534_wholemount_weak_3D_1.wlz
4534_wholemount_possible_3D_1.wlz
4534_wholemount_notDetected_3D_1.wlz
4534_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4534_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
limb
strong strong
branchial arch
strong strong
embryo
strong strong
regionalStrong expression in the head.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Map2k5 probeA
Entity Detected:Map2k5, mitogen-activated protein kinase kinase 5 ( MGI:1346345)
Sequence:sense strand is shown

>Map2k5 probeA
AGGATTCGCCGGTCCTTCCGCTTGGAGAGTTCTCGGAGCCGTTTGTACATTTCATCACTCAGTGCATGAG
GAAGCAGCCCAAGGAGAGACCGGCGCCTGAGGAGCTGATGGGTCACCCATTCATCGTGCAGTTCAATGAT
GGAAACTCCACTGTGGTGTCCATGTGGGTGTGCCGAGCTCTAGAGGAGAGGCGGAGCCAGCAGGGACCCC
CGTGAGACCTCAGCAGGACACTGACCACTGAGGACCAGTCATCATGACACAGCAAGCCGTGACCCCTCTG
TCTGCTGTACTGGCCCAGCCTCCTGCTCGCACCTCCTGCCCTACCTAGGAAGCCCTCCAGAGGGCCAGCC
TCTGCTGAGCGGGGGAAGTGGGCACTGACTACAGCAGGTGGAGATGGTGCAGGGCGGGGTGATGAGGTTA
TAAAACTATGGAACCTCCTCTGGCCCCACCCCCTTATCTTTCCCTACTGTTCATTGTAAAGGGTCAGGCC
CTATCAGCAGCACTGAT
nt 1728 - nt 2234 of NM_011840.2
Notes:The Map2k5 (MKK5) probe used in this study by Yan et al., 2003. [PMID:14675480] is described as thus: "Probe for MKK5 (corresponding to the last 71 amino acid and first 295 bp of the 3' utr) was generated by PCR using the primers ACTAGTAGGATTCGCCGGTCCTTC and ATCAGTGCTGCTGATAGGGCCTGAC. PCR products were cloned into pBluescript to give antisense sequence when transcribed from the T7 promoter".
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Yan L; Carr J; Ashby PR; Murry-Tait V; Thompson C; Arthur JS. 2003. [PMID:14675480] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1186/1471-213X-3-11] [ PMID:14675480] Yan L, Carr J, Ashby PR, Murry-Tait V, Thompson C, Arthur JS 2003 Knockout of ERK5 causes multiple defects in placental and embryonic development. BMC Dev Biol (3):11
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE