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EMAGE:4536

Mapk1 mitogen-activated protein kinase 1 ( MGI:1346858)
TS18 (11.5 dpc)
immunohistochemistry

Data Images
EMAGE:4536
Fig 1I. Copyright: This image is from Li C; Scott DA; Hatch E; Tian X; Mansour SL. "Dusp6 (Mkp3) is a negative feedback regulator of FGF-stimulated ERK signaling during mouse development." Development 2007;134:167-76 [doi:10.1242/dev.02701] and is displayed with the permission of The Company of Biologist Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annnotations: ba, branchial arches; lb, limb bud; op, olfactory pit; ov, otic vesicle.
Expression Pattern Description
Spatial Annotation:
EMAGE:4536Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4536_wholemount_moderate_3D_1.wlz
4536_wholemount_weak_3D_1.wlz
4536_wholemount_possible_3D_1.wlz
4536_wholemount_notDetected_3D_1.wlz
4536_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4536_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
limb
detected detected
branchial arch
detected detected
olfactory pit
detected detected
otocyst
detected detected
Expressed in otic vesicle.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:2677398
Entity Detected:Mapk1, mitogen-activated protein kinase 1 ( MGI:1346858)
Notes:Diphosphorylated (dp) MAPK1 and MAPK3 (ERK2 and ERK1) in this study by Li et al., 2007 [PMID:17164422] were detected "with antibodies directed against diphosphorylated ERK1/2 exactly as described by Corson et al., 2003 [PMID:12925581] ". Editors Note: Corson et al describe the use of two dpERK1/2 antibodies - a rabbit polyclonal and a mouse monoclonal. They state: "Two different primary antibodies against the diphosphorylated forms of ERK1 and ERK2 were used in this study. The first phospho-ERK antibody was a mouse monoclonal antibody (#M8159, Sigma) raised against a synthetic diphosphorylated peptide corresponding to highly conserved residues around Thr183/Tyr185 of ERK2. The second phospho-ERK antibody was a rabbit polyclonal antibody raised against a similar (if not identical) peptide (#9101, Cell Signaling Technology). The two antibodies gave essentially the same staining patterns in mouse except in regions where endogenous mouse immunoglobulins reside. Mouse monoclonal antibody staining is problematic in such regions owing to the crossreactivity of secondary anti-mouse antibodies to the endogenous immunoglobulins found in the tissue. For this reason, the rabbit polyclonal antibody was used in the majority of these studies". Ed: It is assumed that Li et al., 2007 [PMID:17164422] used the rabbit polyclonal (Phospho-p44/442 MAP Kinase (Thr202/Tyr204) antibody).
Antibody Type:polyclonal
Raised In:rabbit
Supplier:Cell Signaling Technology
Catalogue Number:9101
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:8% paraformaldehyde
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Li C; Scott DA; Hatch E; Tian X; Mansour SL. 2007. [PMID:17164422] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.02701] [ PMID:17164422] Li C, Scott DA, Hatch E, Tian X, Mansour SL 2007 Dusp6 (Mkp3) is a negative feedback regulator of FGF-stimulated ERK signaling during mouse development. Development (134):167-76
 [ doi:10.1242/dev.00669] [ PMID:12925581] Corson LB, Yamanaka Y, Lai KM, Rossant J 2003 Spatial and temporal patterns of ERK signaling during mouse embryogenesis. Development (130):4527-37
Links:EMAGE:4537 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE