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EMAGE:4539

Mcm5 minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae) ( MGI:103197)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4539
Fig 5A (+/+). [doi:10.1007/s004270050163] Hill AL; Phelan SA; Loeken MR. "Reduced expression of pax-3 is associated with overexpression of cdc46 in the mouse embryo." 1998. Dev Genes Evol 208:128-134.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Expression in wild-type embryos is weak, and increases progressively in strength in Sp/+ and Sp/Sp embryos (see Fig5A (+/Sp) and (Sp/Sp) therein).
Expression Pattern Description
Spatial Annotation:
EMAGE:4539Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4539_wholemount_weak_3D_1.wlz
4539_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4539_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
neural tube
weak weak
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Mcm5 probeA
Entity Detected:Mcm5, minichromosome maintenance deficient 5, cell division cycle 46 (S. cerevisiae) ( MGI:103197)
Notes:The Mcm5 (cdc46) probe used in this study by Hill et al., 1998 [PMID:9601985] is described as "a digoxigenin-labeled riboprobe complementary to a 274-bp sequence of murine cdc46 (AP5G2)".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6J
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Hill AL, Phelan SA, Loeken MR. 1998 [PMID:9601985] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1007/s004270050163] [ PMID:9601985] Hill AL, Phelan SA, Loeken MR 1998 Reduced expression of pax-3 is associated with overexpression of cdc46 in the mouse embryo. Dev Genes Evol (208):128-34
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE