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EMAGE:454

Tgfb2 transforming growth factor, beta 2 ( MGI:98726)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:454
Figure 3K of Millan et al., 1991 [PMID:1707784] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: p - precartilaginous blastema. It is not stated in text whether image is of a forelimb or a hindlimb.
Expression Pattern Description
Spatial Annotation:
EMAGE:454Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
454_voxel_strong_3D_1.wlz
454_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:454_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
limb
detected detected
regionalHigh levels in precartilaginous blastemae.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1342960
Entity Detected:Tgfb2, transforming growth factor, beta 2 ( MGI:98726)
Sequence:sense strand is shown

>MGI:1342960
AGGAGAAGGCAAGCCGGAGGGCAGCCGCCTGCGAGCGCGAGCGGAGCGAGCAGGAGTACTACGCCAAGGA
GGTTTATAAAATCGACATGCCGTCCCACCTCCCCTCCGAAAATGCCATCCCGCCCACTTTCTACAGACCC
TACTTCAGAATCGTCCGCTTTGATGTCTCAACAATGGAGAAAAATGCTTCGAATCTGGTGAAGGCAGAGT
TCAGGGTCTTCCGCTTGCAAAACCCCAAAGCCAGAGTGGCCGAGCAGCGGATTGAACTGTATCAGATCCT
TAAATCCAAAGACTTAACATCTCCCACCCAGCGCTACATCGATAGCAAGGTTGTGAAAACCAGAGCGGAG
GGTGAATGGCTCTCCTTCGACGTGACAGACGCTGTGCAGGAGTGGCTTCACCACAAAGACAGGAACCTGG
GGTTTAAAATAAGTTTACACTGCCCCTGCTGTACCTTCGTGCCGTCTAATAATTACATCATCCCGAATAA
AAGCGAAGAGCT
nt 1453 - nt 1954 of X57413.1
Notes:The Tgfb2 probe used in this study by Millan et al., 1991 [PMID:1707784] is described as "a 501 nucleotide PstI-SacI fragment, spanning amino acid residues 81 to 249."
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:Parkes x NIH
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Millan et al., 1991 [PMID:1707784] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1707784] Millan FA, Denhez F, Kondaiah P, Akhurst RJ 1991 Embryonic gene expression patterns of TGF beta 1, beta 2 and beta 3 suggest different developmental functions in vivo. Development (111):131-43
Links:MGI:2136195 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE