EMAGE mouse gene expression spatial database

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EMAGE:4540

Mdm2 transformed mouse 3T3 cell double minute 2 ( MGI:96952)
TS14 (14-16 Somite no.)
in situ hybridisation

Data Images


Fig 1I Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(01)00339-2] Mech Dev 103: 163-165, Daujat S; Neel H; Piette J, Preferential expression of Mdm2 oncogene during the development of neural crest and its derivatives in mouse early embryogenesis. Copyright 2001	Fig 1K Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(01)00339-2] Mech Dev 103: 163-165, Daujat S; Neel H; Piette J, Preferential expression of Mdm2 oncogene during the development of neural crest and its derivatives in mouse early embryogenesis. Copyright 2001	Fig 2A Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(01)00339-2] Mech Dev 103: 163-165, Daujat S; Neel H; Piette J, Preferential expression of Mdm2 oncogene during the development of neural crest and its derivatives in mouse early embryogenesis. Copyright 2001	Fig 2B Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(01)00339-2] Mech Dev 103: 163-165, Daujat S; Neel H; Piette J, Preferential expression of Mdm2 oncogene during the development of neural crest and its derivatives in mouse early embryogenesis. Copyright 2001	Fig 2C Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(01)00339-2] Mech Dev 103: 163-165, Daujat S; Neel H; Piette J, Preferential expression of Mdm2 oncogene during the development of neural crest and its derivatives in mouse early embryogenesis. Copyright 2001

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

1I shows a specimen stained with antisense probe and 1K, a similarly staged embryo stained with a sense probe. The three sections 2A, 2B and 2C are taken from the embryo shown in 1I: rostral to caudal cryosections (A-C). Image annotations: 1,2,3 - streams of neural crest cells extending towards the first, second and third branchial arches (labeled 1-3, respectively); ec, ectoderm; fg, foregut; me, mesenchyme; nf, neural fold.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4540_wholemount_moderate_3D_1.wlz

4540_wholemount_weak_3D_1.wlz

4540_wholemount_possible_3D_1.wlz

4540_wholemount_notDetected_3D_1.wlz

4540_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4540_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

1st branchial arch mesenchyme derived from neural crest

detected

Crest cells migrating into 1st branchial arch are most likely derived from rhombomere 2

2nd branchial arch mesenchyme derived from neural crest

detected

Crest cells migrating into 2nd branchial arch are most likely derived from rhombomere 4

3rd branchial arch mesenchyme derived from neural crest

detected

Crest cells migrating into 3rd branchial arch are most likely derived from rhombomere 6

rhombomere 02

detected

regional

Crest cells migrating into 1st branchial arch are most likely derived from rhombomere 2

rhombomere 04

detected

regional

Crest cells migrating into 2nd branchial arch are most likely derived from rhombomere 4

rhombomere 06

detected

regional

Crest cells migrating into 3rd branchial arch are most likely derived from rhombomere 6

head mesenchyme derived from neural crest

detected

Expression is detected in craniofacial mesenchyme

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Mdm2 probeA
Entity Detected:	Mdm2, transformed mouse 3T3 cell double minute 2 ( MGI:96952)
Sequence:	sense strand is shown >Mdm2 probeA ATGTGCAATACCAACATGTCTGTACCTACTGATGGTGCTGTAACCACCTCACAGATTCCAGCTTCGGAAC AAGAGACCCTGGTTAGACCAAAGCCATTGCTTTTGAAGTTATTAAAGTCTGTTGGTGCACAAAAAGACAC TTATACTATGAAAGAGGTTCTTTTTTATCTTGGCCAGTATATTATGACTAAACGATTATATGATGAGAAG CAACAACATATTGTATATTGTTCAAATGATCTTCTAGGAGATTTGTTTGGCGTGCCAAGCTTCTCTGTGA AAGAGCACAGGAAAATATATACCATGATCTACAGGAACTTGGTAGTAGTCAATCAGCAGGAATCATCGGA CTCAGGTACATCTGTGAGTGAGAACAGGTGTCACCTTGAAGGTGGGAGTGATCAAAAGGACCTTGTACAA GAGCTTCAGGAAGAGAAACCTTCATCTTCACATTTGGTTTCTAGACCATCTACCTCATCTAGAAGGAGAG CAATTAGTGAGACAGAAGAAAATTCAGATGAATTATCTGGTGAACGACAAAGAAAACGCCACAAATCTGA TAGTATTTCCCTTTCCTTTGATGAAAGCCTGGCTCTGTGTGTAATAAGGGAGATATGTTGTGAAAGAAGC AGTAGCAGTGAATCTACAGGGACGCCATCGAATCCGGATCTTGATGCTGGTGTAAGTGAACATTCAGGTG ATTGGTTGGATCAGGATTCAGTTTCAGATCAGTTTAGTGTAGAATTTGAAGTTGAATCTCTCGACTCAGA AGATTATAGCCTTAGTGAAGAAGGACAAGAACTCTCAGATGAAGATGATGAGGTATATCAAGTTACTGTG TATCAGGCAGGGGAGAGTGATACAGATTCATTTGAAGAAGATCCTGAAATTTCCTTAGCTGACTATTGGA AATGCACTTCATGCAATGAAATGAATCCCCCCCTTCCATCACATTGCAACAGATGTTGGGCCCTTCGTGA GAATTGGCTTCCTGAAGATAAAGGGAAAGATAAAGGGGAAATCTCTGAGAAAGCCAAACTGGAAAACTCA ACACAAGCTGAAGAGGGCTTTGATGTTCCTGATTGTAAAAAAACTATAGTGAATGATTCCAGAGAGTCAT GTGTTGAGGAAAATGATGATAAAATTACACAAGCTTCACAATCACAAGAAAGTGAAGACTATTCTCAGCC ATCAACTTCTAGTAGCATTATTTATAGCAGCCAAGAAGATGTGAAAGAGTTTGAAAGGGAAGAAACCCAA GACAAAGAAGAGAGTGTGGAATCTAGTTTGCCCCTTAATGCCATTGAACCTTGTGTGATTTGTCAAGGTC GACCTAAAAATGGTTGCATTGTCCATGGCAAAACAGGACATCTTATGGCCTGCTTTACATGTGCAAAGAA GCTAAAGAAAAGGAATAAGCCCTGCCCAGTATGTAGACAACCAATTCAAATGATTGTGCTAACTTATTTC CCCTAG
	nt 1 - nt 1476 of A61359.1
Notes:	The Mdm2 probe used in this study by Daujat et al., 2001 [PMID:11335127] is described as such: "riboprobes correspond to the complete 1.5 kb Mdm2 cDNA fragment; they were isolated from plasmid pXJ-Mdm2 (gift from B. Wasylyk) and cloned in pBluescript KS+". Editors note: searching of GenBank finds a 1476 bp Mdm2 fragment from B.Wasylyk which covers the entire ORF and is assumed to correspond to this clone.
Chemistry:	RNA
Strand:	antisense

Specimen

Organism:	mouse
Strain:	involves: B6D2
Age:	14-16 Somite no.
Theiler Stage:	TS14
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Daujat S, Neel H, Piette J. 2001 [PMID:11335127] . Indexed and spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/S0925-4773(01)00339-2] [ PMID:11335127] Daujat S, Neel H, Piette J 2001 Preferential expression of Mdm2 oncogene during the development of neural crest and its derivatives in mouse early embryogenesis. Mech Dev (103):163-5
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source