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EMAGE:4542

Med15 mediator complex subunit 15 ( MGI:2137379)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4542
Fig 6B. [doi:10.1006/geno.2001.6566] Berti L; Mittler G; Przemeck GK; Stelzer G; Gunzler B; Amati F; Conti E; Dallapiccola B; Hrabe de Angelis M; Novelli G; Meisterernst M. "Isolation and characterization of a novel gene from the digeorge chromosomal region that encodes for a mediator subunit." Genomics 2001 Jun;74(3):320-32.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4542Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4542_wholemount_moderate_3D_1.wlz
4542_wholemount_possible_3D_1.wlz
4542_wholemount_notDetected_3D_1.wlz
4542_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4542_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
fronto-nasal process
detected detected
1st branchial arch
detected detected
2nd branchial arch
detected detected
forelimb bud
detected detected
hindlimb bud
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Med15 probeA
Entity Detected:Med15, mediator complex subunit 15 ( MGI:2137379)
Notes:The Med15 (Pcqap) probe used in this study by Berti et al., 2001 [PMID:11414760] is described by the authors both as "a 528-bp NotI BamHI fragment of Pcqap subcloned into pBluescript" and as "bp 78 to bp 606 of the mouse Pcqap cDNA". Editors note: Elsewhere in the manuscript the authors refer to AF328770 as the identifier for this sequence. One BamHI (at position 2059) and no NotI sites are found in AF328770.1. The exact sequence covered by this probe is unclear from this information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C3H
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + unspecified reagent from Roche
General Information
Authors:Berti L; Mittler G; Przemeck GK; Stelzer G; Gunzler B; Amati F; Conti E; Dallapiccola B; Hrabe de Angelis M; Novelli G; Meisterernst M. 2001 [PMID:11414760] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/geno.2001.6566] [ PMID:11414760] Berti L, Mittler G, Przemeck GK, Stelzer G, Ganzler B, Amati F, Conti E, Dallapiccola B, Hrabe de Angelis M, Novelli G, Meisterernst M 2001 Isolation and characterization of a novel gene from the DiGeorge chromosomal region that encodes for a mediator subunit. Genomics (74):320-32
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE