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EMAGE:4543

Med24 mediator complex subunit 24 ( MGI:1344385)
TS15 (9.5 dpc)
in situ reporter

Data Images
EMAGE:4543
Fig 1D-E9.5 [doi:10.1093/emboj/cdf348] Ito M; Okano HJ; Darnell RB; Roeder RG. 2002. EMBO J. 21:3464-3475. The TRAP100 component of the TRAP/Mediator complex is essential in broad transcriptional events and development. © Nature Publishing Group. Reproduced with permission from EMBO J.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4543Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4543_wholemount__moderate.wlz
4543_wholemount__possible.wlz
4543_wholemount__notDetected.wlz
4543_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4543_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
limb
strong strong
biliary bud intrahepatic part
strong strong
central nervous system
strong strong
tail central nervous system
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Med24, mediator complex subunit 24 ( MGI:1344385)
Notes:Med24 (Trap100) expression in this study by Ito et al., 2002 [PMID:12093747] was assayed in heterozygote embryos where the endogenous locus was disrupted by the in frame insertion of lacZ and neo immediately after the translational start codon. Normal transcript was undetected by Northern blot analysis of homozygous mutant mice. Whole mount staining of mutant embryos indicated that the expression of lacZ was driven by the endogenous promoter.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:involves: 129X1/SvJ * C57BL/6
Age:9.5 dpc
Theiler Stage:TS15
Mutations:Med24tm1Rgr/Med24+
Preparation:wholemount
Procedures
General Information
Authors:Ito M; Okano HJ; Darnell RB; Roeder RG. 2002. [PMID:12093747] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1093/emboj/cdf348] [ PMID:12093747] Ito M, Okano HJ, Darnell RB, Roeder RG 2002 The TRAP100 component of the TRAP/Mediator complex is essential in broad transcriptional events and development. EMBO J (21):3464-75
Links:MGI:3604023 same experiment
 MGI:2446465 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE