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EMAGE:4550

Melk maternal embryonic leucine zipper kinase ( MGI:106924)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4550
Fig3c. Copyright: This image is from 3.0.CO;2-H [doi:10.1002/(SICI)1097-0177(199908)215:4344::AID-AJA6>3.0.CO;2-H] Heyer BS; Kochanowski H; Solter D, "Expression of Melk, a new protein kinase, during early mouse development." Dev Dyn 1999 Aug;215(4):344-51, and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotation: arrow points to the ventral portion of the somite.
Expression Pattern Description
Spatial Annotation:
EMAGE:4550Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4550_wholemount_moderate_3D_1.wlz
4550_wholemount_weak_3D_1.wlz
4550_wholemount_possible_3D_1.wlz
4550_wholemount_notDetected_3D_1.wlz
4550_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4550_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
tail
detected detected
At E9.5, Melk expression is observed in the tail region, where it is restricted to the ventral portion of the somites (Fig. 3C). From this location cells will later migrate in a medioventral direction towards the notochord and will form the axial skeleton.
trunk somite
detected detected
regionalAt E9.5, Melk expression is observed in the tail region, where it is restricted to the ventral portion of the somites (Fig. 3C). From this location cells will later migrate in a medioventral direction towards the notochord and will form the axial skeleton.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Melk probeA
Entity Detected:Melk, maternal embryonic leucine zipper kinase ( MGI:106924)
Notes:The Melk probe used in this study by Heyer et al., 1999 [PMID:10417823] is described as follows "The entire Melk cDNA clone was subcloned in pBluescript KS vector (Stratagene, La Jolla, CA), linearized with MluI and transcribed as an antisense riboprobe using the T3 RNA polymerase". Editors Note: The identity or length of this "entire Melk cDNA clone" is not reported.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:B6D2F1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde (pH 7.4)
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Heyer BS; Kochanowski H; Solter D. 1999 [PMID:10417823] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/(SICI)1097-0177(199908)215:4<344::AID-AJA6>3.0.CO;2-H] [ PMID:10417823] Heyer BS, Kochanowski H, Solter D 1999 Expression of Melk, a new protein kinase, during early mouse development. Dev Dyn (215):344-51
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE