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EMAGE:4557

Mll3 myeloid/lymphoid or mixed-lineage leukemia 3 ( MGI:2444959)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4557
Fig 2c [doi:10.1016/j.gene.2005.11.013] Brun ME; Gasca S; Girard C; Bouton K; De Massy B; De Sario A, Characterization and expression analysis during embryo development of the mouse ortholog of MLL3. 2006 Gene 371(1): 25-33.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4557Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4557_wholemount_moderate.wlz
4557_wholemount_weak.wlz
4557_wholemount_possible.wlz
4557_wholemount_notDetected.wlz
4557_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4557_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
heart
not detected not detected
homogeneous
future forebrain
strong strong
lens placode
detected detected
Expression in optic placode
1st branchial arch
detected detected
neural tube
detected detected
regionalexpressed ventrally
forelimb bud
detected detected
trunk somite
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Mll3 probeA
Entity Detected:Mll3, myeloid/lymphoid or mixed-lineage leukemia 3 ( MGI:2444959)
Sequence:sense strand is shown

>Mll3 probeA
GCAATGTCCCTTGTGGCCAAGACCCTGCTGTAACACAGGGACAGAATTATTCAGGATCCAGCCAGTCTCT
CATTCAGTTATATTCTGACATAATTCCAGAAGAAAAGGGGAAAAAGAAAAGAACAAGAAAAAAGAAAAAA
GATGATGATGCAGAATCCGGCAAAGCACCGTCAACTCCCCACTCTGACTGCGCTGCTCCACTAACCCCAG
GCCTCTCAGAAACTACCTCCACTCCTGCGGTGAGCTCACCCAGTGAGCTCCCTCAGCAAAGACAACAGGA
GCCGGTGGGGCCAGTGCCTGTACCCACTCCAAATGTGTCAGTAGGCCAGCCTTGCATAGAGTCAGAAAAC
AAACTTCCCAACAGTGAATTCATAAAAGAAACTTCAAATCAACAAACACATGTGAATGCAGAGGCAGACA
AGCCTTCCGTGGAAACCCCTAACAAAACTGAAGAAATAAAGTTGGAAAAGGCTGAGACACAGCCAAGTCA
GGAGGATACCAAAGTGGAAGAGAAAACTGGTAATAAGATCAAAGACATTGTAGCTGGTCCTGTCTCCTCA
ATACAGTGTCCTTCCCATCCTGTCGGAACCCCTACTACCAAAGGAGATACAGGAAATGAGCTATTGAAGC
ACTTGTTAAAAAATAAGAAGGCCTCTTCCCTTCTAACTCAGAAACCTGAGGGCACTTTATCTTCAGATGA
AAGTTCTACAAAGGATGGTAAACTGATTGAGAAGCAGAGTCCAGCAGAAGGATTGCAGAATAATTTAAGT
AATCCTCCAACACCCCCTGCCTCTCTTCCTCCTACACCACCTCCTATGGCTTGCCAGAAGATGGCAAATG
nt 6746 - nt 7585 of AY138582.1
Notes:The Mll3 probe used in this study by Brun et al., 2006 [PMID:16459028] was transcribed from either a clone with a "840 bp-insert (from the 3'end of Mll3)" containing "nucleotides 6746 to 7585 from the Mll3 transcript (Genbank Accession no. AY138582)" or "another 1 kb-template (clone 2 that contained nucleotides 1525 to 2745) corresponding to the 5' end of Mll3". Editors Note: The authors state that "the results obtained with the two probes were similar" and they do not stipulate which was used in each assay.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldhyde/PBT
General Information
Authors:Brun ME; Gasca S; Girard C; Bouton K; De Massy B; De Sario A. 2006 [PMID:16459028] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.gene.2005.11.013] [ PMID:16459028] Brun ME, Gasca S, Girard C, Bouton K, De Massy B, De Sario A 2006 Characterization and expression analysis during embryo development of the mouse ortholog of MLL3. Gene (371):25-33
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE