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EMAGE:4563

Mstn myostatin ( MGI:95691)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4563
Fig1C. Copyright: Reprinted with permission from Cell Press from [doi:10.1016/S0896-6273(02)01172-8] Neuron 37: 197-207, Wu HH; Ivkovic S; Murray RC; Jaramillo S; Lyons KM; Johnson JE; Calof AL, Autoregulation of Neurogenesis by GDF11. Copyright 2003

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4563Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4563_wholemount_moderate.wlz
4563_wholemount_notDetected.wlz
4563_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4563_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
nasal epithelium
not detected not detected
homogeneous
trunk somite
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Mstn probeA
Entity Detected:Mstn, myostatin ( MGI:95691)
Sequence:sense strand is shown

>Mstn probeA
AGCCATGGTAGTAGACCGCTGTGGGTGCTCATGAGCTTTGCATTAGGTTAGAAACTTCCCAAGTCATGGA
AGGTCTTCCCCTCAATTTCGAAACTGTGAATTCAAGCACCACAGGCTGTAGGCCTTGAGTATGCTCTAGT
AACGTAAGCACAAGCTACAGTGTATGAACTAAAAGAGAGAATAGATGCAATGGTTGGCATTCAACCACCA
AAATAAACCATACTATAGGATGTTGTATGATTTCCAGAGTTTTTGAAATAGATGGAGATCAAATTACATT
TATGTCCATATATGTATATTACAACTACAATCTAGGCAAGGAAGTGAGAGCACATCTTGTGGTCTGCTGA
GTTAGGAGGGTATGATTAAAAGGTAAAGTCTTATTTCCTAACAGTTTCACTTAATATTTACAGAAGAATC
TATATGTAGCCTTTGTAAAGTGTAGGATTGTTATCATTTAAAAACATCATGTACACTTATATTTGTATTG
TATACTTGGTAAGATAAAATTCCACAAAGTAGGAATGGGGCCTCACATACACATTGCCATTCCTATTATA
ATTGGACAATCCACCACGGTGCTAATGCAGTGCTGAATGGCTCCTACTGGACCTCTCGATAGAACACTCT
ACAAA
nt 1201 - nt 1835 of NM_010834.1
Notes:The Mstn (Gdf8) probe used in this study by Wu et al., 2003 [PMID:12546816] covered "bases 1201-1835 of mouse Gdf8 3' UTR (GenBank #NM010834)". Editors note: when this paper was published in 2003, only one version of NM_010834 existed (NM_010834.1).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Wu HH; Ivkovic S; Murray RC; Jaramillo S; Lyons KM; Johnson JE; Calof AL. 2003 [PMID:12546816] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0896-6273(02)01172-8] [ PMID:12546816] Wu HH, Ivkovic S, Murray RC, Jaramillo S, Lyons KM, Johnson JE, Calof AL 2003 Autoregulation of neurogenesis by GDF11. Neuron (37):197-207
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE