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EMAGE:4579

Epha2 Eph receptor A2 ( MGI:95278)
TS17 (11 dpc)
in situ hybridisation

Data Images
EMAGE:4579 EMAGE:4579
Figure 5E. Copyright: This image is from Moore-Scott BA et al., [doi:10.1002/dvdy.21204] Dev Dyn 2007 Jul;236(7):1997-2003 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright. Figure 5E'. Copyright: This image is from Moore-Scott BA et al., [doi:10.1002/dvdy.21204] Dev Dyn 2007 Jul;236(7):1997-2003 and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: section plane of 5E' is indicated on 5E; hg - hindgut.
Expression Pattern Description
Spatial Annotation:
EMAGE:4579Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4579_wholemount_moderate_3D_1.wlz
4579_wholemount_weak_3D_1.wlz
4579_wholemount_possible_3D_1.wlz
4579_wholemount_notDetected_3D_1.wlz
4579_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4579_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
tail
detected detected
hindgut
detected detected
forelimb bud mesenchyme
detected detected
regionalExpression is in a domain of the limb mesenchyme beneath the apical ectodermal ridge.
hindlimb bud mesenchyme
detected detected
regionalExpression is in a domain of the limb mesenchyme beneath the apical ectodermal ridge.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3715948
Entity Detected:Epha2, Eph receptor A2 ( MGI:95278)
Sequence:sense strand is shown

>MGI:3715948
ATTCCTATCTGAGTCCATTGGGGCCGTGCCCAACAATACTTGAAGAGCCACAGTGGTCTCCCTGCCGACC
TGGTGCTGGCCCACTGGAACTTTATTTATTTCTGTTTCCTCGTCTATGCCTCCCTGAGGACTCTGCAGGG
GGCTTTTGAATGACACCCTGGCCTGAGCCTGGGAAACTTGGATGCTGGTCAGGGCTCTCTTTCCCCTGCA
AAGGACCCAGCTAAGCACTTAGCAGTTTGCCATGGCCTTCCCAGCATCCCCTGAGGCTAAAGTTCCACCA
AGACCGTCGATATCGACGAGGGACATTTCCAAACGGACCTCCCCATCTTCATTTGGCCTCCTGAGAAGCC
ACCTCAGGAGCTGAGGCTAAGCACTAAGCCCAGGACCATATGACTAGGGCACTGTACGCCCCGCCCCTAG
TTAGAGGGTAGGTTTTGGACTTGGCTGGGTGTGGTCACAGCAATCTCCCAGTGCCTTTTACAGACCCAGG
GCTCTGCCCTCCGCCCTCGAGGGCCAGCTTCTTGCTTTCCTAGGGCCCTCTCAGGATGCTTGGCTGTGCT
GAGG
nt 3036 - nt 3599 of NM_010139.2
Notes:The Epha2 probe used in this study by Moore-Scott et al., 2007 [PMID:17576135] was generated by RT-PCR using the following primers: "F: 5'-gga att cct atc tga gtc cat-3' 3010F; R: 5'-cct cag cac agc caa gca t-3' 3565R".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:11 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:methanol
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Moore-Scott BA; Opoka R; Lin SC; Kordich JJ; Wells JM, 2007 [PMID:17576135] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.21204] [ PMID:17576135] Moore-Scott BA, Opoka R, Lin SC, Kordich JJ, Wells JM 2007 Identification of molecular markers that are expressed in discrete anterior-posterior domains of the endoderm from the gastrula stage to mid-gestation. Dev Dyn (236):1997-2003
Links:MGI:3716120 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI