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EMAGE:458

Tgfb3 transforming growth factor, beta 3 ( MGI:98727)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:458
Figure 3L of Millan et al., 1991 [PMID:1707784] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: p - precartilaginous blastema. It is not stated in the text whether image is of a forelimb or hindlimb.
Expression Pattern Description
Spatial Annotation:
EMAGE:458Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
458_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:458_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
limb
not detected not detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1342961
Entity Detected:Tgfb3, transforming growth factor, beta 3 ( MGI:98727)
Sequence:sense strand is shown

>MGI:1342961
GTCCTGGCCCTGCTGAACTTGGCCACAATCAGCCTCTCTCTGTCCACTTGCACCACGTTGGACTTCGGCC
ACATCAAGAAGAAGAGGGTGGAAGCCATTAGGGGACAGATCTTGAGCAAGCTCAGGCTCACCAGCCCCCC
TGAGCCATCGGTGATGACCCACGTCCCCTATCAGGTCCTGGCACTTTACAACAGCACCCGGGAGTTGCTG
GAAGAGATGCACGGGGAGAGGGAGGAAGGCTGCACTCAGGAGACCTCGGAGTCTGAGTACTATGCCAAAG
AGATCCATAAATTCGACATGATCCAGGGACTGGCGGAGCACAATGAACTGGCCGTCTGCCCCAAAGGAAT
TACCTCTAAGGTTTTTCGTTTCAATGTGTCCTCAGTGGAGAAAAATGGAACCAATCTGTTCCGGGCAGAG
TTCCGGGTCTTGCGGGTGCCCAACCCCAGCTCCAAGCGCACAGAGCAGAGAATTGAGCTCTTCCAGATAC
TTCGACCGGATGAGCACATAGCCAAGCAGCGCTACATAGGTGGCAAGAATCTGCCCACAAGGGGCACCGC
TGAATGGCTGTCTTTCGATGTCACTGACACTGTGCGCGAGTGGCTGTTGAGGAGAGAGTCCAACTTGGGT
CTGGAAATCAGCATCCACTGTCCATGTCACACCTTTCAGCCCAATGGAGACATACTGGAAAATGTTCATG
AGGTGATGGAAATCAAATTCAAAGGAGTGGACA
nt 635 - nt 1367 of M32745.1
Notes:The Tgfb3 probe used in this study by Millan et al., 1991 [PMID:1707784] is described as "a 732 nucleotide fragment, spanning amino acid residues 8 to 251 of the precursor polypeptide."
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:Parkes x NIH
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Millan et al., 1991 [PMID:1707784] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1707784] Millan FA, Denhez F, Kondaiah P, Akhurst RJ 1991 Embryonic gene expression patterns of TGF beta 1, beta 2 and beta 3 suggest different developmental functions in vivo. Development (111):131-43
Links:MGI:2136196 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE