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EMAGE:4583

Acta2 actin, alpha 2, smooth muscle, aorta ( MGI:87909)
TS19 (11.5 dpc)
immunohistochemistry

Data Images
EMAGE:4583 EMAGE:4583 EMAGE:4583
Figure 6A. Copyright: This image is from Hallaq H, Development 2004 Oct;131(20):5197-209 [PMID:15459110] and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 6D. Copyright: This image is from Hallaq H, Development 2004 Oct;131(20):5197-209 [PMID:15459110] and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 6G. Copyright: This image is from Hallaq H, Development 2004 Oct;131(20):5197-209 [PMID:15459110] and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Arrows in D highlight the same vascular branches. Arrowheads in G indicate the vascular smooth muscle cells in the dorsal aorta.
Expression Pattern Description
Spatial Annotation:
EMAGE:4583Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4583_wholemount_moderate.wlz
4583_wholemount_notDetected.wlz
4583_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4583_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
cardiovascular system
strong strong
homogeneousExpression is in in blood vessels and is highlighted in the cranial vasculature.
dorsal aorta
strong strong
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1314968
Entity Detected:Acta2, actin, alpha 2, smooth muscle, aorta ( MGI:87909)
Notes:The anti-ACTA2 (alphaSM actin/alpha-SMA) antibody used in this study by Hallaq et al., 2004 [PMID:15459110] was obtained from Sigma and used at a 1:500 dilution.
Antibody Type:monoclonal
Raised In:mouse
Supplier:Sigma
Specimen
Organism:mouse
Strain:involves: 129X1/SvJ * C57BL/6J
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Hallaq H; Pinter E; Enciso J; McGrath J; Zeiss C; Brueckner M; Madri J; Jacobs HC; Wilson CM; Vasavada H; Jiang X; Bogue CW, 2004 [PMID:15459110] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.01393] [ PMID:15459110] Hallaq H, Pinter E, Enciso J, McGrath J, Zeiss C, Brueckner M, Madri J, Jacobs HC, Wilson CM, Vasavada H, Jiang X, Bogue CW 2004 A null mutation of Hhex results in abnormal cardiac development, defective vasculogenesis and elevated Vegfa levels. Development (131):5197-209
Links:MGI:3511114 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI