Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4589

Bmp6 bone morphogenetic protein 6 ( MGI:88182)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4589
Figure 2F. Copyright: This image is from Furuta et al., Development 124:2203-2212 (1997), [PMID:9187146] and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4589Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4589_wholemount_possible_3D_1.wlz
4589_wholemount_strong_3D_1.wlz
4589_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4589_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalExpression is detected through entire body axis along dorsal midline.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:9001
Entity Detected:Bmp6, bone morphogenetic protein 6 ( MGI:88182)
Sequence:sense strand is shown

>MGI:9001
GACGGCCCGCGAGGAGCCCCCTCCAGGGCGGCTGAAGTCCGCTCCACTCTTCATGCTGGATCTCTACAAC
GCCCTGTCCAATGACGACGAAGAGGATGGGGCATCGGAGGGTGTGGGGCAAGAGCCTGGGTCCCACGGAG
GGGCCAGCTCGTCCCAGCTCAGGCAGCCGTCTCCCGGCGCTGCACACTCCTTGAACCGCAAGAGTCTCCT
GGCCCCGGGACCCGGTGGCGGTGCGTCCCCACTGACTAGCGCGCAGGACAGCGCTTTCCTCAACGACGCG
GACATGGTCATGAGCTTTGTGAACCTGGTGGAGTACGACAAGGAGTTCTCCCCACATCAACGACACCACA
AAGAGTTCAAGTTCAACCTATCCCAGATTCCTGAGGGTGAGGCGGTGACGGCTGCTGAGTTCCGCGTCTA
CAAGGACTGTGTGGTGGGGAGTTTTAAAAACCAAACCTTTCTTATCAGCATTTACCAAGTCTTGCAGGAG
CATCAGCACAGAGACTCTGACCTATTTTTGTTGGACACCCGGGTGGTGTGGGCCTCAGAAGAAGGTTGGC
TGGAATTTGACATCACAGCAACTAGCAATCTGTGGGTGGTGACACCGCAGCACAACATGGGGCTCCAGCT
GAGTGTGGTGACTCGGGATGGACTCCACGTCAACCCCCGTGCGGCGGGCCTGGTGGGCAGAGACGGCCCT
TACGACAAGCAGCCCTTCATGGTGGCCTTCTTCAAGGTGAGCGAGGTCCACGTGCGCACCACCAGGTCAG
CCTCCAGTCGGCGGCGGCAGCAGAGTCGCAACCGGTCCACCCAGTCGCAGGACGTGTCCCGGGGCTCCGG
TTCTTCAGACTACAACGGCAGTGAGTTAAAAACAGCTTGCAAGAAGCATGAGCT
nt 564 - nt 1457 of NM_007556.1
Notes:The Bmp6 (Vgr1) probe used in this study by Furuta et al., 1997 [PMID:12930780] was "a 900 bp fragment of Bmp6 (Jones et al., 1991 [PMID:1893873] )". Jones et al. in turn refer to Lyons et al., 1989 [PMID:2481605] who describe "an 893-bp SacI-EcoRI fragment from the 5' region of the Vgr-1 cDNA (Lyons et al., 1989 [PMID:2734307] )." As restriction analysis of Bmp6/Vgr1 clones in the public databases (eg NM_007556.1) finds an internal SacI cut site, but no 5' EcoRI site, this 5' EcoRI site is presumably linker derived. The fragment shown above extends 5' from the SacI site at nt1457 for 893nt.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Furuta Y; Piston DW; Hogan BL, 1997 [PMID:9187146] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9187146] Furuta Y, Piston DW, Hogan BL 1997 Bone morphogenetic proteins (BMPs) as regulators of dorsal forebrain development. Development (124):2203-12
Links:MGI:1276856 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI