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EMAGE:4606

Myl2 myosin, light polypeptide 2, regulatory, cardiac, slow ( MGI:97272)
TS14 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4606
Figure 5L. Copyright: This image is from Roebroek et al., Development 125: 4863-4876 (1998), [PMID:9811571] and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotation: h, heart.
Expression Pattern Description
Spatial Annotation:
EMAGE:4606Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4606_wholemount_notDetected_3D_1.wlz
4606_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4606_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1309867
Entity Detected:Myl2, myosin, light polypeptide 2, regulatory, cardiac, slow ( MGI:97272)
Notes:The Myl2 (MLC2V) probe used in this study by Roebroek et al., 1998 [PMID:9811571] is indicated as that described by Lyons et al., 1995 [PMID::7628699] i.e. "The MLC2V probe was prepared from plasmid described above". Editors note: Elsewhere in the Materials and Methods, Lyons et al describe a plasmid used to generate a probe for an RNAse protection assay: "The MLC2V probe was transcribed with T7 polymerase (Promega) from the BamHI-linearized plasmid pcMLC2V (supplied by Kenneth Chien, University of California, San Diego), a 449-bp partial-length cDNA cloned into pBS.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:involves: 129P2/OlaHsd * C57BL/6
Age:9.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Roebroek AJ; Umans L; Pauli IG; Robertson EJ; van Leuven F; Van de Ven WJ ; Constam DB, 1998 [PMID:9811571] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9811571] Roebroek AJ, Umans L, Pauli IG, Robertson EJ, van Leuven F, Van de Ven WJ, Constam DB 1998 Failure of ventral closure and axial rotation in embryos lacking the proprotein convertase Furin. Development (125):4863-76
 [ doi:10.1101/gad.9.13.1654] [ PMID:7628699] Lyons I, Parsons LM, Hartley L, Li R, Andrews JE, Robb L, Harvey RP 1995 Myogenic and morphogenetic defects in the heart tubes of murine embryos lacking the homeo box gene Nkx2-5. Genes Dev (9):1654-66
Links:MGI:1331444 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE