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EMAGE:4622

Tgfb2 transforming growth factor, beta 2 ( MGI:98726)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4622 EMAGE:4622
Figure 5H. Copyright: This image is from Dickson MC, Development 1993 Feb;117(2):625-39, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 5G. Copyright: This image is from Dickson MC, Development 1993 Feb;117(2):625-39, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: av= atrio-ventricular region, lb= limb bud, lm= lateral plate mesoderm, ot= outflow tract, st, septum transversum; v, ventricle. Scale bar= 160 microns.
Expression Pattern Description
Spatial Annotation:
EMAGE:4622Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4622_wholemount_moderate_3D_1.wlz
4622_wholemount_possible_3D_1.wlz
4622_wholemount_notDetected_3D_1.wlz
4622_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4622_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
atrioventricular canal
detected detected
regionalExpression is more restriceted than in 9.0dpc.
forelimb bud mesenchyme
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1342960
Entity Detected:Tgfb2, transforming growth factor, beta 2 ( MGI:98726)
Sequence:sense strand is shown

>MGI:1342960
AGGAGAAGGCAAGCCGGAGGGCAGCCGCCTGCGAGCGCGAGCGGAGCGAGCAGGAGTACTACGCCAAGGA
GGTTTATAAAATCGACATGCCGTCCCACCTCCCCTCCGAAAATGCCATCCCGCCCACTTTCTACAGACCC
TACTTCAGAATCGTCCGCTTTGATGTCTCAACAATGGAGAAAAATGCTTCGAATCTGGTGAAGGCAGAGT
TCAGGGTCTTCCGCTTGCAAAACCCCAAAGCCAGAGTGGCCGAGCAGCGGATTGAACTGTATCAGATCCT
TAAATCCAAAGACTTAACATCTCCCACCCAGCGCTACATCGATAGCAAGGTTGTGAAAACCAGAGCGGAG
GGTGAATGGCTCTCCTTCGACGTGACAGACGCTGTGCAGGAGTGGCTTCACCACAAAGACAGGAACCTGG
GGTTTAAAATAAGTTTACACTGCCCCTGCTGTACCTTCGTGCCGTCTAATAATTACATCATCCCGAATAA
AAGCGAAGAGCT
nt 1453 - nt 1954 of X57413.1
Notes:The Tgfb2 probe used in this study by Dickson et al., 1993 [PMID:7687212] is indicated as that described by Millan et al., 1991 [PMID:1707784] as "a 501 nucleotide Pstl-Sacl fragment, spanning amino acid residues 81 to 249".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:involves: NIH * Parkes
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dickson MC; Slager HG; Duffie E; Mummery CL; Akhurst RJ, 1993 [PMID:7687212] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7687212] Dickson MC, Slager HG, Duffie E, Mummery CL, Akhurst RJ 1993 RNA and protein localisations of TGF beta 2 in the early mouse embryo suggest an involvement in cardiac development. Development (117):625-39
 [ PMID:1707784] Millan FA, Denhez F, Kondaiah P, Akhurst RJ 1991 Embryonic gene expression patterns of TGF beta 1, beta 2 and beta 3 suggest different developmental functions in vivo. Development (111):131-43
Links:MGI:1343196 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE