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EMAGE:4628

Sept5 septin 5 ( MGI:1195461)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4628
Fig 1A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00370-1] Mech Dev 96: 121-124, Maldonado-Saldivia J; Funke B; Pandita RK; Schuler T; Morrow BE; Schorle H; Expression of Cdcrel-1 (Pnutl1), a gene frequently deleted in velo-cardio-facial syndrome/DiGeorge syndrome. Copyright 2000

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: dorsal root ganglia (drg), vagal (X), glossopharyngeal (IX), facio-acoustic (VII-VIII), and trigeminal (V) cranial ganglia.
Expression Pattern Description
Spatial Annotation:
EMAGE:4628Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4628_wholemount_moderate_3D_1.wlz
4628_wholemount_notDetected_3D_1.wlz
4628_wholemount_strong_3D_1.wlz
4628_wholemount_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4628_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
vagus x ganglion
detected detected
glossopharyngeal ix ganglion
detected detected
facial vii ganglion
detected detected
acoustic viii ganglion
detected detected
trigeminal v ganglion
detected detected
dorsal root ganglion
detected detected
neural tube lateral wall
detected detected
Signals were detected in the lateral layer of the neural tube.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sept5 probeA
Entity Detected:Sept5, septin 5 ( MGI:1195461)
Sequence:sense strand is shown

>Sept5 probeA
GGGAACTGAAGGAAAGTGCACCTTTCGCCGTTATTGGCAGCAACACTGTGGTGGAGGCCAAGGGGCAGCG
GGTCCGGGGGCGACTGTACCCCTGGGGGATCGTCGAAGTGGAGAATCAGGCGCACTGCGACTTTGTGAAG
CTCCGCAACATGCTCATCCGCACTCACATGCACGACCTCAAAGATGTGACGTGCGACGTGCACTATGAGA
ACTACCGTGCCCACTGCATCCAGCAGATGACCAGCAAACTCACCCAGGACAGCCGCATGGAGAGCCCCAT
TCCTATCCTCCCACTACCCACACCGGATGCGGAGACCGAGAAGCTCATCAGGATGA
nt 642 - nt 977 of AF033350.1
Notes:The Sept5 (Cdcrel-1) probe used in this study by Maldonado-Saldivia et al., 2000 [PMID:10940632] is described as follows: "A 351 bp fragment of the murine cDNA spanning bases 642-977 (Genbank accession number AF 033350) was generated by PCR and cloned in the EcoRI site of pBS II (KS)."
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Maldonado-Saldivia J; Funke B; Pandita RK; Schuler T; Morrow BE; Schorle H, 2000 [PMID:10940632] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(00)00370-1] [ PMID:10940632] Maldonado-Saldivia J, Funke B, Pandita RK, Schuler T, Morrow BE, Schorle H 2000 Expression of Cdcrel-1 (Pnutl1), a gene frequently deleted in velo-cardio-facial syndrome/DiGeorge syndrome. Mech Dev (96):121-4
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE