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EMAGE:4629

Sept9 septin 9 ( MGI:1858222)
TS17 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4629
Fig5D. from [doi:10.1016/S0378-1119(02)00406-7] Gene 285: 79-89, Sorensen AB; Warming S; Fuchtbauer EM; Pedersen FS , Alternative splicing, expression, and gene structure of the septin-like putative proto-oncogene Sint1. Copyright 2002

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4629Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4629_wholemount_moderate_3D_1.wlz
4629_wholemount_weak_3D_1.wlz
4629_wholemount_notDetected_3D_1.wlz
4629_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4629_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud
strong strong
hindlimb bud
strong strong
branchial arch
strong strong
olfactory pit
strong strong
Sint1 is strongly expressed in the olfactory placode.
dorsal root ganglion
strong strong
ear
detected detected
Sint1 is expressed around the otic pit.
trunk somite
weak weak
Expression in the somites remains but appears weaker, particularly because there is a strong segmental expression in the neural crest cells that will give rise to the dorsal root ganglia.
tail somite
weak weak
Expression in the somites remains but appears weaker, particularly because there is a strong segmental expression in the neural crest cells that will give rise to the dorsal root ganglia.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Sept9 probeA
Entity Detected:Sept9, septin 9 ( MGI:1858222)
Sequence:sense strand is shown

>Sept9 probeA
AATTCGGCACGAGGCGCCAGAACACAACCGGAGTCCCTGAGGCTCCAGAACAGAGTCAGGGTGAGCCAGC
CCCAGCCCAGCCCCAGGTTCTGGCTTCCTCTCTTGCTTCTCCCTCCTTTGGTTGCAGCCAGCAGAGCCCA
CTTAAACTCCGAGGTGACTCCCAGCTGTGTTGGCGACATGGCTGACAACCCTAGAGATGCCATGCTCAAG
CAGGCCCCCGCGTCACGGAACGAAAAGGCCCCCATGGAGTTCGGCTATGTGGGGATCGACTCCATCCTGG
AGCAGATGCGCAGGAAGGCTATGAAACAGGGCTTCGAGTTTAACATCATGGTGGTTGGGCAGAGCGGCCT
CGGGAAGTCCACTTTAATCAATACCCTCTTCAAGTCCAAAATCAGCCGGAAGTCGGTGCAGCCCACCTCG
GAGGAACGCATCCCCAAGACGATCGAAATCAAGTCGATCACCCACGATATTGAAGAGAAGGGGGTTCGAA
TGAAGCTGACAGTGATTGACACGCCGGGCTTCGGAGACCACATCAACAATGAGAACTGCTGGCAGCCCAT
TATGAAGTTCATCAATGACCAATATGAGAAGTACCTGCAGGAGGAAGTCAACATCAACCGGAAGAAGCGC
ATTCCCGACACCCGTGTCCACTGCTGCCTCTACTTCATCCCAGCCACCGGCCACTCGCTCAGGCCCCTGG
ACATTG
nt 1 - nt 706 of AJ250723.1
Notes:The Sept9 (Sint1) probe used in this study by Sorensen et al., 2002 [PMID:12039034] is described as "Probe 1", "an EcoRI fragment of about 700 bp of Sint1 cDNA clone 1; Accession number: AJ250723." Editors note: EcoRI cuts AJ250723.1 once, 706nt from the 5'end. It is assumed that the other EcoRI site is liker derived. See Fig 1 of Sorensen et al for a schematic representation of the region covered.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Sorensen AB; Warming S; Fuchtbauer EM; Pedersen FS, 2002 [PMID:12039034] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0378-1119(02)00406-7] [ PMID:12039034] Sorensen AB, Warming S, Fuchtbauer EM, Pedersen FS 2002 Alternative splicing, expression, and gene structure of the septin-like putative proto-oncogene Sint1. Gene (285):79-89
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE