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EMAGE:4632

Chst15 carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 ( MGI:1924840)
TS14 (10.0 dpc)
in situ hybridisation

Data Images
EMAGE:4632
Fig2F. from [doi:10.1387/ijdb.062168as] Int J Dev Biol 50: 705-708, Salgueiro AM; Filipe M; Belo JA, N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase expression during early mouse embryonic development. Copyright 2006

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4632Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4632_wholemount_moderate_3D_1.wlz
4632_wholemount_notDetected_3D_1.wlz
4632_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4632_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
detected detected
mesenchyme derived from splanchnopleure
detected detected
vitelline artery
detected detected
future diencephalon roof plate
detected detected
midgut
detected detected
embryo
detected detected
regionalExpression is in the endoderm cells in contact with the somatopleure layer.
1st branchial arch
detected detected
regionalExpression is in the epithelium.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2395037
Entity Detected:Chst15, carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 ( MGI:1924840)
Sequence:sense strand is shown

>MGI:2395037
CGCCTGCGCTGCCTGCCCCACTTCTACATCATTGGGCAGCCCAAGTGTGGAACCACTGACCTGTACGACC
GCCTCCGGCTGCATCCAGAAGTGAAATTCTCAGCCATCAAGGAGCCGCACTGGTGGACCCGGAAGCGCTT
TGGAATTGTCCGCCTGCGGGACGGACTACGAGACCGCTACCCTGTGGAAGATTACCTGGACCTCTTTGAC
TTGGCTGCACACCAGATCCATCAAGGACTGCAGGCTGCCTCTGCAGAGCAGCCCAGCAAGATGAATAAGA
TCATTATTGGAGAGGCCAGCGCCTCTACAATGTGGGATAACAATGCCTGGACCTTCTTCTATGACAACAG
CACAGACGGCGAGCCACCATTCCTGACCCAAGACTTCATCCATGCCTTTCAACCAGAAGCCAAGCTCATT
GTTATGCTCAGGGATCCTGTGGAGAGGTTGTACTCAGACTATNTNTACTTCGCAAGTTCCAATAAATCTG
CAGATGACTTCCACGAGAAAGTGACGGAGGCTCTGCAGCTGTTTGAAAATTGCATGCTGGATTATTCAAT
GCGCGCCTGCGTCTACAACAACACCCTGAACAATGCCATGCCCGTGAGGCTCCAAGTTGGCCTGTATGCT
GTGTACCTTCGGGATTGGCTGGACG
Notes:The 4631426J05Rik (GalNAcS-6ST) probe used in this study by Salgueiro et al., 2006 [PMID:17051481] is described as follows: "The full-length clone of mouse GaINAc4S-6ST (accession number: AV322573), containing the entire coding sequence as well as the 5' and 3'-UTR, was acquired from RZPD (4631426J05Rik). Digoxigenin labeled GaINAc4S-6ST mRNA probe was synthesized by linearizing the AV322573 clone with EcoRI and transcribing with T7 RNA polymerase."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:(C57BL/6 x SJL)F1
Age:10.0 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Salgueiro AM; Filipe M; Belo JA, 2006 [PMID:17051481] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1387/ijdb.062168as] [ PMID:17051481] Salgueiro AM, Filipe M, Belo JA 2006 N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase expression during early mouse embryonic development. Int J Dev Biol (50):705-8
Links:MGI:3691205 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI