Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4635

Acta1 actin, alpha 1, skeletal muscle ( MGI:87902)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4635 EMAGE:4635
Fig1B. Copyright: This image is from [doi:10.1002/dvdy.20176] Golding JP; Partridge TA; Beauchamp JR; King T; Brown NA; Gassmann M; Zammit PS, "Mouse myotomes pairs exhibit left-right asymmetric expression of MLC3F and alpha-skeletal actin." Dev Dyn 2004 Dec;231(4):795-800, and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright. Fig1A. Copyright: This image is from [doi:10.1002/dvdy.20176] Golding JP; Partridge TA; Beauchamp JR; King T; Brown NA; Gassmann M; Zammit PS, "Mouse myotomes pairs exhibit left-right asymmetric expression of MLC3F and alpha-skeletal actin." Dev Dyn 2004 Dec;231(4):795-800, and is displayed with the permission of Wiley-Liss, Inc. who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: ht - heart. Whole-mount in situ hybridisations show increased levels of skeletal actin mRNA on the left side (A), compared with the right side (B)
Expression Pattern Description
Spatial Annotation:
EMAGE:4635Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4635_wholemount_possible.wlz
4635_wholemount_notDetected.wlz
4635_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4635_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalalpha-skeletal actin was expressed throughout the dorsoventral extent of the myotome. It is also noted that it is transiently expressed more strongly within the left myotome of myotome pairs.
heart
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Acta1 probeA
Entity Detected:Acta1, actin, alpha 1, skeletal muscle ( MGI:87902)
Notes:The Acta1 (alpha-skeletal actin) probe used in this study by Golding et al., 2004 [PMID:15499557] is indicated as that previously used by Zammit et al., 2000 [PMID:10679931] , which is of unreported sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/10
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Golding JP; Partridge TA; Beauchamp JR; King T; Brown NA; Gassmann M; Zammit PS, 2004 [PMID:15499557] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.20176] [ PMID:15499557] Golding JP, Partridge TA, Beauchamp JR, King T, Brown NA, Gassmann M, Zammit PS 2004 Mouse myotomes pairs exhibit left-right asymmetric expression of MLC3F and alpha-skeletal actin. Dev Dyn (231):795-800
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE