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EMAGE:4636

Acta2 actin, alpha 2, smooth muscle, aorta ( MGI:87909)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:4636
Fig 4A. Copyright: This image is from [doi:10.1073/pnas.1233635100] Li S; Wang DZ; Wang Z; Richardson JA; Olson EN, Proc Natl Acad Sci U S A 2003 Aug 5;100(16):9366-70. Copyright 2003 National Academy of Sciences, U.S.A.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4636Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4636_wholemount_moderate.wlz
4636_wholemount_notDetected.wlz
4636_wholemount_strong.wlz
4636_wholemount_possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4636_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
dorsal aorta
detected detected
regionalSM alpha-actin-positive SMCs were present in the dorsal aorta and cardinal veins of WT embryos.
cardinal vein
detected detected
regionalSM alpha-actin-positive SMCs were present in the dorsal aorta and cardinal veins of WT embryos.
heart
detected detected
cardiovascular system
detected detected
regionalSM alpha-actin is expressed in the heart and developing vasculature of WT embryos.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:Acta2 antibodyA
Entity Detected:Acta2, actin, alpha 2, smooth muscle, aorta ( MGI:87909)
Antigen:sense strand is shown

>Acta2 antibodyA
EEEDSTALVC
aa 3 - aa 12 of NP_031418.1
Notes:The Acta2 (SM alpha-actin) antibody used in this study by Li et al., 2003 [PMID:12867591] is described as "SM alpha-actin Ab (clone 1A4, Sigma)." The method used was previously described in Lin et al., 1998 [PMID:9778514] . Editors note: There are 5 products Sigma offer that relate to clone 1A4 (A5691, A5228, A2547, C6198, F3777 - all Monoclonal Anti-Actin, alpha-Smooth Muscle antibodies in various conjugated forms) and it is not clear which one was used in this study. However the Sigma product information states that the immunogen is a N-terminal synthetic decapeptide of alpha-smooth muscle actin. See also Skalli et al, 1986 [PMID:3539945] for further production details where the peptide immunogen is described as: Ac-Glu(OBut)-Glu(OBut)-Glu(OBut)-Asp(OBut)-Ser(But)-Thr(But)-Ala-Leu-Val-Cys(Acm)-NHEt.
Antibody Type:monoclonal
Raised In:mouse
Supplier:Sigma
Catalogue Number:clone 1A4
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Li S; Wang DZ; Wang Z; Richardson JA; Olson EN, 2003 [PMID:12867591] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1073/pnas.1233635100] [ PMID:12867591] Li S, Wang DZ, Wang Z, Richardson JA, Olson EN 2003 The serum response factor coactivator myocardin is required for vascular smooth muscle development. Proc Natl Acad Sci U S A (100):9366-70
 [ PMID:9778514] Lin Q, Lu J, Yanagisawa H, Webb R, Lyons GE, Richardson JA, Olson EN 1998 Requirement of the MADS-box transcription factor MEF2C for vascular development. Development (125):4565-74
 [ doi:10.1083/jcb.103.6.2787] [ PMID:3539945] Skalli O, Ropraz P, Trzeciak A, Benzonana G, Gillessen D, Gabbiani G 1986 A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. J Cell Biol (103):2787-96
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE