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EMAGE:4640

Adh5 alcohol dehydrogenase 5 (class III), chi polypeptide ( MGI:87929)
TS14 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4640
Fig4H. from [PMID:8621625] J Biol Chem 271: 9526-9534, Ang HL; Deltour L; Hayamizu TF; Zgombic-Knight M; Duester G, Retinoic acid synthesis in mouse embryos during gastrulation and craniofacial development linked to class IV alcohol dehydrogenase gene expression. Copyright 1996

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4640Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4640_wholemount_moderate_3D_1.wlz
4640_wholemount_weak_3D_1.wlz
4640_wholemount_notDetected_3D_1.wlz
4640_wholemount_strong_3D_1.wlz
4640_wholemount_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4640_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Authors report nearly ubiquitous expression at all stages analyzed.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:30904
Entity Detected:Adh5, alcohol dehydrogenase 5 (class III), chi polypeptide ( MGI:87929)
Notes:The Adh5 (class III ADH) probe used in this study by Ang et al., 1996 [PMID:8621625] is described as "prepared from full-length cDNA."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:FVB/N
Age:9.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase
General Information
Authors:Ang HL; Deltour L; Hayamizu TF; Zgombic-Knight M; Duester G, 1996 [PMID:8621625] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1074/jbc.271.16.9526] [ PMID:8621625] Ang HL, Deltour L, Hayamizu TF, Zgombic-Knight M, Duester G 1996 Retinoic acid synthesis in mouse embryos during gastrulation and craniofacial development linked to class IV alcohol dehydrogenase gene expression. J Biol Chem (271):9526-34
Links:MGI:2384738 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI