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EMAGE:4641

Adh7 alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide ( MGI:87926)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4641
Fig2H. from [PMID:8621625] J Biol Chem 271: 9526-9534, Ang HL; Deltour L; Hayamizu TF; Zgombic-Knight M; Duester G, Retinoic acid synthesis in mouse embryos during gastrulation and craniofacial development linked to class IV alcohol dehydrogenase gene expression. Copyright 1996

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4641Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4641_wholemount_moderate_3D_1.wlz
4641_wholemount_weak_3D_1.wlz
4641_wholemount_notDetected_3D_1.wlz
4641_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4641_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
regionalAuthors report expression in posterior trunk.
forelimb bud
detected detected
head mesenchyme
detected detected
regionalExpression in the head limited to ventral craniofacial structures (eg. frontonasal mass, perioptic region).
1st branchial arch mandibular component
detected detected
1st branchial arch maxillary component
detected detected
2nd branchial arch
detected detected
future brain
possible possible
homogeneousexpression was absent or low in all regions of the brain.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2384730
Entity Detected:Adh7, alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide ( MGI:87926)
Notes:The Adh7 (class IV ADH) probe used in this study by Ang et al., 1996 [PMID:8621625] is described as "prepared from full-length cDNA."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:FVB/N
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase
General Information
Authors:Ang HL; Deltour L; Hayamizu TF; Zgombic-Knight M; Duester G, 1996 [PMID:8621625] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1074/jbc.271.16.9526] [ PMID:8621625] Ang HL, Deltour L, Hayamizu TF, Zgombic-Knight M, Duester G 1996 Retinoic acid synthesis in mouse embryos during gastrulation and craniofacial development linked to class IV alcohol dehydrogenase gene expression. J Biol Chem (271):9526-34
Links:MGI:2384736 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI