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EMAGE:4643

Adnp activity-dependent neuroprotective protein ( MGI:1338758)
TS16 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4643
Fig 2d from [doi:10.1016/S0165-3806(03)00162-7] Pinhasov A; Mandel S; Torchinsky A; Giladi E; Pittel Z; Goldsweig AM; Servoss SJ; Brenneman DE; Gozes I. 2003 Brain Res Dev Brain Res 2003 Aug 12;144(1):83-90 [PMID:12888219]

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4643Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4643_wholemount_moderate_3D_1.wlz
4643_wholemount_possible_3D_1.wlz
4643_wholemount_notDetected_3D_1.wlz
4643_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4643_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future brain
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3712130
Entity Detected:Adnp, activity-dependent neuroprotective protein ( MGI:1338758)
Sequence:sense strand is shown

>MGI:3712130
ACCTGCAGCAAAACAACTATGGAGTCAAATCTGTGGGCCAGAGCTATGGTGTTGGCCAGTCAGTGAGGCT
GGGACTAGGTGGCAATGCTCCAGTTTCCATCCCTCAACAGTCTCAGTCCGTGAAACAGTTACTTCCAAGT
GGGAATGGGAGGTCTTTTGGGCTAGGTGCTGAGCAGAGGCCCCCAGCAGCAGCCAGGTACTCCCTGCAGA
CTGCCAACACCTCTCTACCCCCAGGCCAAGTGAAGTCTCCCTCTGTGTCTCAGTCACAGGCATCTAGAGT
ATTAGGTCAGTCCAGTTCTAAACCTCCACCAGCCGCCACAGGCCCTCCTCCAAGCAACCACTGTGCCACT
CAGAAGTGGAAAATCTGTACAATCTGTAACGAGC
nt 137 - nt 520 of AF068198.1
Notes:The Adnp probe used in this study by Pinhasov et al., 2003 [PMID:12888219] is described as follows: "Digoxigenin-labeled (Roche, Mannheim, Germany) cRNA probe to ADNP was obtained following subcloning into pGEM-EASY (Promega, Madison, WI, USA). The ADNP cDNA was obtained by reverse transcription-polymerase chain reaction (RT-PCR) (upper primer 5'-ACCTGCAGCAAAACAACTAT-3' ; lower primer 5'-GCTCGTTACAGATTGTAC-3' ) (Bassan et al., 1999 [PMID:10037502] )."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Pinhasov A; Mandel S; Torchinsky A; Giladi E; Pittel Z; Goldsweig AM; Servoss SJ; Brenneman DE; Gozes I, 2003 [PMID:12888219] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0165-3806(03)00162-7] [ PMID:12888219] Pinhasov A, Mandel S, Torchinsky A, Giladi E, Pittel Z, Goldsweig AM, Servoss SJ, Brenneman DE, Gozes I 2003 Activity-dependent neuroprotective protein: a novel gene essential for brain formation. Brain Res Dev Brain Res (144):83-90
 [ doi:10.1046/j.1471-4159.1999.0721283.x] [ PMID:10037502] Bassan M, Zamostiano R, Davidson A, Pinhasov A, Giladi E, Perl O, Bassan H, Blat C, Gibney G, Glazner G, Brenneman DE, Gozes I 1999 Complete sequence of a novel protein containing a femtomolar-activity-dependent neuroprotective peptide. J Neurochem (72):1283-93
Links:MGI:3712141 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI