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EMAGE:4650

Ankrd1 ankyrin repeat domain 1 (cardiac muscle) ( MGI:1097717)
TS15 (9.0 dpc)
in situ hybridisation

Data Images
EMAGE:4650
Fig 4C. Copyright: This image is from Zou Y; Evans S; Chen J; Kuo HC; Harvey RP; Chien KR, Development 1997;124(4):793-804 [PMID:9043061] , and is displayed with the permission of The Company of Biologist Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: a, atrial; v, ventricle; ct, conotruncus.
Expression Pattern Description
Spatial Annotation:
EMAGE:4650Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4650_wholemount_moderate_3D_1.wlz
4650_wholemount_notDetected_3D_1.wlz
4650_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4650_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
common atrial chamber
strong strong
carp was specifically and evenly expressed at a high level in the looping heart tube throughout the common atrial chamber, the common ventricular chamber and the outflow tract.
primitive ventricle
strong strong
carp was specifically and evenly expressed at a high level in the looping heart tube throughout the common atrial chamber, the common ventricular chamber and the outflow tract.
bulbus cordis
strong strong
carp was specifically and evenly expressed at a high level in the looping heart tube throughout the common atrial chamber, the common ventricular chamber and the outflow tract.
outflow tract
strong strong
carp was specifically and evenly expressed at a high level in the looping heart tube throughout the common atrial chamber, the common ventricular chamber and the outflow tract.
heart
detected detected
regionalExpression of carp is uniform throughout all cardiac muscle compartments.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Ankrd1 probeA
Entity Detected:Ankrd1, ankyrin repeat domain 1 (cardiac muscle) ( MGI:1097717)
Sequence:sense strand is shown

>Ankrd1 probeA
GGCTGCCAACATGATGGTTTTTCGAGTAGAGGAGCTGGTAACGGGCAAAAAGAACAGCAATGGGTCCTCA
GGGGAGTTCCTTCCTGGCGAGTTCAGAAATGGAGAGTATGAAGCTGCTGTTGCCTTGGAGAAACAAGAGG
ACTTGAAGACACTTCCAGCCAACAGTGTGAACCTGGGGGAAGAACAACGGAAAAGTGAGAAAGTTCGAGA
GGCAGAGCTCAAAAAGAAAAAACTAGAACAAAGATCAAAGCTTGAAAACTTAGAAGACCTTGAAATAATT
GTTCAACTGAAGAAAAGAAAAAAATACAAGAAAACTAAAGTTCCAGTTGTGAAGGAACCGGAGCCTGAAA
TTATTACTGAACCTGTGGATGTGCCAAGGTTTCTGAAAGCTGCACTGGAGAACAAACTGCCAGTTGTAGA
GAAATTCCTGTCAGACAAGAACAGCCCCGACGTCTGCGATGAGTATAAACGGACCGCTCTCCATAGAGCA
TGCTTAGAAGGACACTTGGCGATCGTGGAGAAGTTAATGGAGGCTGGAGCCCAGATTGAATTCCGAGATA
TGCTGGAATCCACAGCCATCCACTGGGCATGTCGTGGAGGAAACCTGGATGTCCTGAAACTGTTGCTAAA
CAAAGGTGCCAAAATCAGTGCCCGAGACAAGCTGCTCAGCACAGCGCTGCACGTGGCGGTGAGGACCGGT
CACTACGAGTGTGCTGAGCACCTCATCGCCTGCGAGGCGGATCTCAATGCCAAGGACAGAGAAGGAGACA
CCCCTCTGCATGATGCGGTGAGGTTGAATCGCTACAAGATGATCCGGCTCTTGATGACCTTCGGTGCGGA
CCTCAATGTCAAGAACTGTGCTGGGAAGACCCCTATGGATCTGGTGTTGCACTGGCAGAATGGAACCAAA
GCGATATTCGACAGCCTCAAGGAGAATGCCTACAAAAACTCGCGCATAGCTACGTTCTGAGGAAAGAGAC
TCGACAGGAGCTGTTCTCAGCCGTTTTCAAAACATTTCCCAGTC
nt 1 - nt 1024 of L81174.1
Notes:The Ankrd1 (Carp) probe used in this study by Zou et al., 1997 [PMID:9043061] is described as follows: "The nucleotide sequence of the mouse and rat carp cDNA was 97% identical, including the 5' and 3' noncoding region. The 1700 base pair rat carp fragment was subcloned into pRC/CMV via a NotI site in the sense and antisense orientations downstream of the T7 promoter. The template constructs were linearized with XbaI. Digoxigenin labeling was performed according to Boehringer Mannheim. Alkaline hydrolysis was carried out to reduce the probe size to an average of 250 base pairs". Editors note: L81174.1 correpsonds to the rat cDNA sequence shown in Fig 1A of Zou et al., 1997. XbaI does not cut this sequence and is therefore assumed to cut in the vector.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.0 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Zou Y; Evans S; Chen J; Kuo HC; Harvey RP; Chien KR, 1997 [PMID:9043061] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9043061] Zou Y, Evans S, Chen J, Kuo HC, Harvey RP, Chien KR 1997 CARP, a cardiac ankyrin repeat protein, is downstream in the Nkx2-5 homeobox gene pathway. Development (124):793-804
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE