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EMAGE:4655

Arl4a ADP-ribosylation factor-like 4A ( MGI:99437)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4655
Fig2C. from [doi:10.1074/jbc.M002470200] J Biol Chem 275 (48): 37815-37823, Lin CY; Huang PH; Liao WL; Cheng HJ; Huang CF; Kuo JC; Patton WA; Massenburg D; Moss J; Lee FJ, ARL4, an ARF-like protein that is developmentally regulated and localized to nuclei and nucleoli. Copyright 2000

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4655Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4655_wholemount_moderate_3D_1.wlz
4655_wholemount_weak_3D_1.wlz
4655_wholemount_notDetected_3D_1.wlz
4655_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4655_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future midbrain
detected detected
regionalmARL4 mRNA was concentrated at the junction of midbrain and hindbrain.
future hindbrain
detected detected
regionalmARL4 mRNA was concentrated at the junction of midbrain and hindbrain.
somite 05
not detected not detected
homogeneousBy this stage, expression has disappeared from the first 10-12 pairs of somites
somite 06
not detected not detected
homogeneousBy this stage, expression has disappeared from the first 10-12 pairs of somites
somite 07
not detected not detected
homogeneousBy this stage, expression has disappeared from the first 10-12 pairs of somites
somite 08
not detected not detected
homogeneousBy this stage, expression has disappeared from the first 10-12 pairs of somites
somite 09
not detected not detected
homogeneousBy this stage, expression has disappeared from the first 10-12 pairs of somites
somite 10
not detected not detected
homogeneousBy this stage, expression has disappeared from the first 10-12 pairs of somites
somite 11
not detected not detected
homogeneousBy this stage, expression has disappeared from the first 10-12 pairs of somites
trunk somite
detected detected
regionalmARL4 mRNA was concentrated in the caudal somites.
future forebrain
detected detected
regionalmARL4 mRNA was concentrated at the junction of forebrain and midbrain.
somite 12
not detected not detected
homogeneousBy this stage, expression has disappeared from the first 10-12 pairs of somites
somite 13
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 14
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 15
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 16
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 17
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 18
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 19
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 20
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 21
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 22
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 23
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 24
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 25
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 26
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 27
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 28
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 29
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
somite 30
detected detected
Expression is not present in the first 10-12 pairs of somites, but is present in the caudal somites
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Arl4a probeA
Entity Detected:Arl4a, ADP-ribosylation factor-like 4A ( MGI:99437)
Notes:The Arl4a probe used in this study by Kuo et al., 2000 [PMID:10980193] is described as follows: "mARL4 RNA probes were prepared from pCRII-mARL4 constructed by PCR amplification of the adult mouse ARL4 cDNA and cloning of the product into the pCRII vector."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Lin CY; Huang PH; Liao WL; Cheng HJ; Huang CF; Kuo JC; Patton WA; Massenburg D; Moss J; Lee FJ, 2000 [PMID:10980193] , Indexed and Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1074/jbc.M002470200] [ PMID:10980193] Lin CY, Huang PH, Liao WL, Cheng HJ, Huang CF, Kuo JC, Patton WA, Massenburg D, Moss J, Lee FJ 2000 ARL4, an ARF-like protein that is developmentally regulated and localized to nuclei and nucleoli. J Biol Chem (275):37815-23
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE