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EMAGE:4659

Apoe apolipoprotein E ( MGI:88057)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4659
Fig2B. from [PMID:10221153] Biofactors 1999 9(1): 11-17, Bachner D; Schroder D; Betat N; Ahrens M; Gross G, Apolipoprotein E (ApoE), a Bmp-2 (bone morphogenetic protein) upregulated gene in mesenchymal progenitors (C3H10T1/2), is highly expressed in murine embryonic development. Copyright 1999.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4659Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4659_wholemount_moderate_3D_1.wlz
4659_wholemount_notDetected_3D_1.wlz
4659_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4659_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
lung
strong strong
High expression is shown in the lung anlagen.
septum transversum hepatic component
strong strong
High expression is shown in the liver.
heart
strong strong
High expression is shown in the heart.
eye
strong strong
High expression is shown in the eye.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Apoe probeA
Entity Detected:Apoe, apolipoprotein E ( MGI:88057)
Notes:The Apoe probe used in this study by Bachner et al., 1999 [PMID:10221153] is of unreported sequence. ISH method of Rosen & Beddington [PMID:8337752] used.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Bachner D; Schroder D; Betat N; Ahrens M; Gross G, 1999 [PMID:10221153] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/biof.5520090103] [ PMID:10221153] Bachner D, Schroder D, Betat N, Ahrens M, Gross G 1999 Apolipoprotein E (ApoE), a Bmp-2 (bone morphogenetic protein) upregulated gene in mesenchymal progenitors (C3H10T1/2), is highly expressed in murine embryonic development. Biofactors (9):11-7
 [ doi:10.1016/0168-9525(93)90162-B] [ PMID:8337752] Rosen B, Beddington RS 1993 Whole-mount in situ hybridization in the mouse embryo: gene expression in three dimensions. Trends Genet (9):162-7
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE