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EMAGE:466

Tcf15 transcription factor 15 ( MGI:104664)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:466
Figure 5K of Koizumi et al., 2001 [PMID:11262239] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:466Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
466_wholemount_strong_3D_1.wlz
466_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:466_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
tail paraxial mesenchyme
detected detected
trunk paraxial mesenchyme
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1334537
Entity Detected:Tcf15, transcription factor 15 ( MGI:104664)
Sequence:sense strand is shown

>MGI:1334537
CTCGGCTGGCGGCCCCACTCCAGTCCGAACACATGTGCCCGCCTCACCCGGCAGCCGCCACCGCGGGGCG
CAGCGGAGACCCCTGGCCCGGCGGCCTGTGGGGACCTAAAGAGGCGGCATGAGCAGCGCACGGTGGAGCT
GACGCCGCGCCCCCTGCCCGGCCCATGTCCTTCGCCATGCTGCGTTCAGCGCCGCCGCCGGGTCGCTACC
TGTACCCTGAGGTGAGCCCGCTGTCGGAGGATGAGGACCGCGGAAGCGAGAGCTCGGGCTCCGACGAGAA
ACCCTGCCGTGTGCATGCTGCGCGCTGTGGCCTCCAGGGCGCCCGGCGGCGGGCAGGAGGACGGAGGGCC
GCGGGTAGCGGGCCAGGACCCGGGGGGCGGCCAGGCCGCGAGCCCCGGCAGCGGCACACAGCGAATGCGC
GCGAGCGGGACCGCACCAACAGCGTGAACACGGCCTTCACTGCGCTGCGCACACTCATCCCCACCGAGCC
AGCGGACCGCAAGCTCTCCAAGATTGAGACGCTGCGCCTGGCCTCCAGCTACATTTCTCACCTGGGCAAT
GTGCTGCTGGTGGGTGAGGCCTGTGGCGACGGGCAACCATGCCACTCGGGACCCGCTTTCTTCCACAGCG
GTCGTGCGGGCAGCCCACTGCCACCGCCGCCGCCGCCACCACCACTGGCCAGAGACGGCGGCGAGAACAC
CCAGCCCAAACAGATCTGCACCTTCTGCCTCAGCAACCAGAGAAAGTTGAGCAAAGACCGTGACAGAAAG
ACGGCGATTCGAAGTTAGAAGGAGGAGGGTCCACAGCAGCCAAGAGGTGATGCCACTAGTGAAGGTGGAT
GCCACTGAAGAGTCACGGAGAGCCCCACTTTGGACCTGATCTGAGCGCAGGTCCCCAAGAGCACGCTCAC
CAGGCCAGACCTGCCCCAGCTATGAGCAGGGCCTATGAACAGAGAGATGGACAGGCGGGTGATGGCTTGA
CTCTAAGCAGCCGAACACTGACCCAGGCCCACTGGGACTTTCCATGCTGGCTCTCTACCTGGGCCAAGTG
TTGGCATCTTGTGTCTTTAATATGATAATATAAAGTCTGGAAATTTTGTATAATTAAAAATGAAACAGTA
TCTTCCAAAAAAAAAAAAAA
nt 1 - nt 1140 of S78079.1
Notes:The Tcf15 (paraxis) probe used in this study by Koizumi et al., 2001 [PMID:11262239] was previously described by Burgess et al., 1995 [PMID:7729571] as "a 1.2-kb paraxis cDNA insert (Cserjesi et al, 1995 [PMID:7743923] ) ". The nucleotide sequence of this clone is depicted in Fig1A of Cserjesi et al. and in GenBank entry S78079
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + unspecified
General Information
Authors:Koizumi et al., 2001 [PMID:11262239] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11262239] Koizumi K, Nakajima M, Yuasa S, Saga Y, Sakai T, Kuriyama T, Shirasawa T, Koseki H 2001 The role of presenilin 1 during somite segmentation. Development (128):1391-402
 [ doi:10.1006/dbio.1995.1081] [ PMID:7729571] Burgess R, Cserjesi P, Ligon KL, Olson EN 1995 Paraxis: a basic helix-loop-helix protein expressed in paraxial mesoderm and developing somites. Dev Biol (168):296-306
 [ PMID:7743923] Cserjesi P, Brown D, Ligon KL, Lyons GE, Copeland NG, Gilbert DJ, Jenkins NA, Olson EN 1995 Scleraxis: a basic helix-loop-helix protein that prefigures skeletal formation during mouse embryogenesis. Development (121):1099-110
Links:MGI:1932858 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI