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EMAGE:4660

Apoe apolipoprotein E ( MGI:88057)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4660
Fig2A. from [PMID:10221153] Biofactors 1999 9(1): 11-17, Bachner D; Schroder D; Betat N; Ahrens M; Gross G, Apolipoprotein E (ApoE), a Bmp-2 (bone morphogenetic protein) upregulated gene in mesenchymal progenitors (C3H10T1/2), is highly expressed in murine embryonic development. Copyright 1999.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4660Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4660_wholemount_moderate_3D_1.wlz
4660_wholemount_weak_3D_1.wlz
4660_wholemount_notDetected_3D_1.wlz
4660_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4660_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
spinal ganglion
strong strong
single cellHigh expression is visible in single cells in spinal ganglia.
future spinal cord
strong strong
single cellHigh expression is visible in cells lining the whole spinal cord.
trunk somite
strong strong
High expression is visible in somites at the hindbrain region.
embryo
strong strong
single cellHigh expression is visible in single cells within most tissues except the CNS.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Apoe probeA
Entity Detected:Apoe, apolipoprotein E ( MGI:88057)
Notes:The Apoe probe used in this study by Bachner et al., 1999 [PMID:10221153] is of unreported sequence. Method of Rosen & Beddington [PMID:8337752] used.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Bachner D; Schroder D; Betat N; Ahrens M; Gross G, 1999 [PMID:10221153] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/biof.5520090103] [ PMID:10221153] Bachner D, Schroder D, Betat N, Ahrens M, Gross G 1999 Apolipoprotein E (ApoE), a Bmp-2 (bone morphogenetic protein) upregulated gene in mesenchymal progenitors (C3H10T1/2), is highly expressed in murine embryonic development. Biofactors (9):11-7
 [ doi:10.1016/0168-9525(93)90162-B] [ PMID:8337752] Rosen B, Beddington RS 1993 Whole-mount in situ hybridization in the mouse embryo: gene expression in three dimensions. Trends Genet (9):162-7
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE