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EMAGE:4664

Asxl1 additional sex combs like 1 (Drosophila) ( MGI:2684063)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4664
Fig5A. from [doi:10.1016/j.gene.2005.10.033] [PMID:16412590] Gene 369: 109-118, Fisher CL; Randazzo F; Humphries RK; Brock HW, Characterization of Asxl1, a murine homolog of Additional sex combs, and analysis of the Asx-like gene family. Copyright 2005

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4664Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4664_wholemount_moderate_3D_1.wlz
4664_wholemount_weak_3D_1.wlz
4664_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4664_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
strong strong
The strongest signal was seen in the telencephalic vesicles, branchial arches, forelimb and hindlimb buds, dorsal root ganglia, and the tail bud.
forelimb bud
strong strong
The strongest signal was seen in the telencephalic vesicles, branchial arches, forelimb and hindlimb buds, dorsal root ganglia, and the tail bud.
hindlimb bud
strong strong
The strongest signal was seen in the telencephalic vesicles, branchial arches, forelimb and hindlimb buds, dorsal root ganglia, and the tail bud.
tail
strong strong
regionalThe strongest signal was seen in the telencephalic vesicles, branchial arches, forelimb and hindlimb buds, dorsal root ganglia, and the tail bud.
dorsal root ganglion
strong strong
The strongest signal was seen in the telencephalic vesicles, branchial arches, forelimb and hindlimb buds, dorsal root ganglia, and the tail bud.
trunk paraxial mesenchyme
detected detected
regionalA lesser degree of expression was observed in the paraxial mesoderm.
heart
possible possible
minimal to no detectable signal in the heart and liver primordia.
liver
possible possible
minimal to no detectable signal in the heart and liver primordia.
embryo
detected detected
regionalAlmost ubiquitous expression of Asxl1 (was detected) in most embryonic tissues.
tail paraxial mesenchyme
detected detected
regionalA lesser degree of expression was observed in the paraxial mesoderm.
lateral ventricle
strong strong
The strongest signal was seen in the telencephalic vesicles, branchial arches, forelimb and hindlimb buds, dorsal root ganglia, and the tail bud.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3656290
Entity Detected:Asxl1, additional sex combs like 1 (Drosophila) ( MGI:2684063)
Sequence:sense strand is shown

>MGI:3656290
GGATCCTATTAAGGCAGATGCAGAGAACAGAAAAGCTGCAGGGTACAGTTCTCTGGAACTAGTGGGTCAC
TTGCAAGGGATGCCTTTTGTTGTGGATCTGCCTTTCTGGAAGTTACCCAGAGAGCCAGGGAAAGGGTTCA
GTCAACCCCTGGAGCCTTCTTCCATCCCTTCCCAACTCAACATCAAGCAGGCCTTGTATGGGAAGTTGTC
TAAACTTCAGCTCAGTCCCACCAGCTTTAATTACTCCTCTAGCTCTGCTACCTTTCCCAAAGGCCTTGCT
GGTGGTGTGGTGCAGCTGAGCCACAAAGCCAGCTTTGGTACAGGCCACACTGCATCACTGTCCTTACAAA
TGTTCGCTGACAGCAGTGCAGTAGAAAGCATCTCTCTCCAATGTGCATGCAGCCTGAAAGCCATGATCAT
GTGCCAAGGCTGCGGAGCATTCTGCCATGATGACTGCATTGGACCTTCAAAGCTCTGTGTATTGTGCCTT
GTGGTGAGATAATAAATTATGGCCATTGGAAACATTGTACATTTAGTGTGTGTATTTTAATAATGGTTGA
TCTTAAATCTGTATACAAAATATCACTGATATAATGAACTCTCTCTCTCTAGACAAGATAAATTTTGCCT
CCCCATGAGATTTATAGTGCTGAAGCCCTCTGTCACTTGACACCCTTCTAGCCTTGTTGGAAGGGTTTTC
AGGGAGATGGGGGCACTATGGTTGCCCAAGACCATAAACCCTCTTGTAGTCAGACAGTATAGTGTAGCAG
GGCAATCTGTCTGACACCTAAATGGACTTGAAATTGAAGCAGGAAGGTTGGGTTCTCCATGGATGGAACT
CACCTGCCTGAACTGAGCAGGAATGTCAGTCTTCCACTGCCCCTCCCTGCCATCTTCTGCTACTTAGCTT
GGGAGTTGATGGTTGCAGAAGCCACACAGGGTTAAAGTAAATTCTGTCTTTGCCCACCAGGGGATCAAAC
CCCTGCTGATCTTGAT
nt 4239 - nt 5234 of NM_001039939.1
Notes:The Asxl1 probe used in this study by Fisher et al., 2006 [PMID:16412590] is described as follows: The Asxl1 riboprobe construct was generated by inserting the 995 bp BamHI-EcoRV fragment of Asxl1 into the pBSKS vector. The plasmid was linearized with NotI and the antisense transcript synthesized with T3 RNA polymerase (Invitrogen)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Fisher CL; Randazzo F; Humphries RK; Brock HW, 2006 [PMID:16412590] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.gene.2005.10.033] [ PMID:16412590] Fisher CL, Randazzo F, Humphries RK, Brock HW 2006 Characterization of Asxl1, a murine homolog of Additional sex combs, and analysis of the Asx-like gene family. Gene (369):109-18
Links:MGI:3656374 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI