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EMAGE:4673

Barhl2 BarH-like 2 (Drosophila) ( MGI:1859314)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4673 EMAGE:4673
Fig 1E. Copyright: This image is from Saba R; Johnson JE; Saito T, Development 2005;132(9):2147-55 [doi:10.1242/dev.01781] and is displayed with the permission of The Company of Biologist Limited who owns the Copyright. [PMID:15788459] Fig 1F. Copyright: This image is from Saba R; Johnson JE; Saito T, Development 2005;132(9):2147-55 [doi:10.1242/dev.01781] and is displayed with the permission of The Company of Biologist Limited who owns the Copyright. [PMID:15788459]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: Arrowheads indicate the ventral telencephalon, dorsal diencephalon and mesencephalon, which expressed Mbh1. Fig1E is a lateral view, Fig1F is a dorsal view.
Expression Pattern Description
Spatial Annotation:
EMAGE:4673Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4673_wholemount_moderate.wlz
4673_wholemount_possible.wlz
4673_wholemount_notDetected.wlz
4673_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4673_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
regionalMbh1 was expressed in several areas of the developing nervous system, including the ventral telencephalon, diencephalon, mesencephalon, hindbrain and spinal cord.
diencephalon
detected detected
Mbh1 was expressed in several areas of the developing nervous system, including the ventral telencephalon, diencephalon, mesencephalon, hindbrain and spinal cord.
midbrain
detected detected
regionalMbh1 was expressed in several areas of the developing nervous system, including the ventral telencephalon, diencephalon, mesencephalon, hindbrain and spinal cord.
hindbrain
detected detected
Mbh1 was expressed in several areas of the developing nervous system, including the ventral telencephalon, diencephalon, mesencephalon, hindbrain and spinal cord.
future spinal cord
detected detected
Mbh1 was expressed in several areas of the developing nervous system, including the ventral telencephalon, diencephalon, mesencephalon, hindbrain and spinal cord. At E11.5, Mbh1 expression extended ventrally to the deep dorsal horn in the spinal cord.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Barhl2 probeA
Entity Detected:Barhl2, BarH-like 2 (Drosophila) ( MGI:1859314)
Notes:The Barhl2 (Mbh1) probe used in this study by Saba et al., 2005 [PMID:15788459] is described as follows, "Antisense RNA probes were synthesized from plasmids carrying mouse cDNA clones: pMH4-1 and pNH10 for Mbh1." Editors Note: The regions covered by either of these clones is not discussed by the authors, nor is it not reported whether the probe was generated from either a mixture of both or just one in different experiments.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Saba R; Johnson JE; Saito T, 2005 [PMID:15788459] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.01781] [ PMID:15788459] Saba R, Johnson JE, Saito T 2005 Commissural neuron identity is specified by a homeodomain protein, Mbh1, that is directly downstream of Math1. Development (132):2147-55
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE