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EMAGE:4678

Bmp2 bone morphogenetic protein 2 ( MGI:88177)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4678
Fig6Ewt. Copyright: This image is from Ybot-Gonzalez P, Development 2007;134(17):3203-11 [doi:10.1242/10.1242/dev.008177] and is displayed with the permission of The Company of Biologist Limited who owns the Copyright. [PMID:17693602]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: Red arrowhead - Bmp2 expression in the dorsal surface ectoderm.
Expression Pattern Description
Spatial Annotation:
EMAGE:4678Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4678_wholemount_moderate_3D_1.wlz
4678_wholemount_possible_3D_1.wlz
4678_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4678_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
surface ectoderm
detected detected
regionalBmp2 expression was detected in the surface ectoderm overlying the (posterior) neural folds.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Bmp2 probeB
Entity Detected:Bmp2, bone morphogenetic protein 2 ( MGI:88177)
Notes:The Bmp2 probe used in this study by Ybot-Gonzalez et al., 2007 [PMID:17693602] is indicated as that previously described by Furuta et al., 1997 [PMID:9187146] i.e. "a 1.2 kb fragment of Bmp2 (Lyons et al., 1989 [PMID:2481605] )." Lyons et al., used a Bmp2 (BMP-2a) probe that was "a 1124-bp EcoRI fragment from the murine BMP-2a cDNA (Dickinson et al, in prep), subcloned into pGEM-3Zf(-) and linearized with XmaIIl. This construct yields a 1-kb riboprobe." Editors note: From this description, it is unclear if the probe used by Ybot-Gonzalez et al., is 1.2, 1.1 or 1kb in length.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:CD-1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Ybot-Gonzalez P; Gaston-Massuet C; Girdler G; Klingensmith J; Arkell R; Greene ND; Copp AJ, 2007 [PMID:17693602] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.008177] [ PMID:17693602] Ybot-Gonzalez P, Gaston-Massuet C, Girdler G, Klingensmith J, Arkell R, Greene ND, Copp AJ 2007 Neural plate morphogenesis during mouse neurulation is regulated by antagonism of Bmp signalling. Development (134):3203-11
 [ PMID:9187146] Furuta Y, Piston DW, Hogan BL 1997 Bone morphogenetic proteins (BMPs) as regulators of dorsal forebrain development. Development (124):2203-12
 [ doi:10.1101/gad.3.11.1657] [ PMID:2481605] Lyons KM, Pelton RW, Hogan BL 1989 Patterns of expression of murine Vgr-1 and BMP-2a RNA suggest that transforming growth factor-beta-like genes coordinately regulate aspects of embryonic developm Genes Dev (3):1657-68
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE