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EMAGE:4680

Bmp6 bone morphogenetic protein 6 ( MGI:88182)
TS18 (11 dpc)
in situ hybridisation

Data Images
EMAGE:4680
Fig1G. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0284] Dev Biol 235(2): 449-66, Kim RY; Robertson EJ; Solloway MJ, Bmp6 and Bmp7 are required for cushion formation and septation in the developing mouse heart. Copyright 2001 [PMID:11437450]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: arrow indicates expression in a small patch of mesenchyme at the base of the forelimb bud.
Expression Pattern Description
Spatial Annotation:
EMAGE:4680Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4680_wholemount_moderate_3D_1.wlz
4680_wholemount_weak_3D_1.wlz
4680_wholemount_notDetected_3D_1.wlz
4680_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4680_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud mesenchyme
detected detected
regionalBmp6 is expressed in a small patch of mesenchyme at the rostral base of the forelimb bud.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:9001
Entity Detected:Bmp6, bone morphogenetic protein 6 ( MGI:88182)
Sequence:sense strand is shown

>MGI:9001
GACGGCCCGCGAGGAGCCCCCTCCAGGGCGGCTGAAGTCCGCTCCACTCTTCATGCTGGATCTCTACAAC
GCCCTGTCCAATGACGACGAAGAGGATGGGGCATCGGAGGGTGTGGGGCAAGAGCCTGGGTCCCACGGAG
GGGCCAGCTCGTCCCAGCTCAGGCAGCCGTCTCCCGGCGCTGCACACTCCTTGAACCGCAAGAGTCTCCT
GGCCCCGGGACCCGGTGGCGGTGCGTCCCCACTGACTAGCGCGCAGGACAGCGCTTTCCTCAACGACGCG
GACATGGTCATGAGCTTTGTGAACCTGGTGGAGTACGACAAGGAGTTCTCCCCACATCAACGACACCACA
AAGAGTTCAAGTTCAACCTATCCCAGATTCCTGAGGGTGAGGCGGTGACGGCTGCTGAGTTCCGCGTCTA
CAAGGACTGTGTGGTGGGGAGTTTTAAAAACCAAACCTTTCTTATCAGCATTTACCAAGTCTTGCAGGAG
CATCAGCACAGAGACTCTGACCTATTTTTGTTGGACACCCGGGTGGTGTGGGCCTCAGAAGAAGGTTGGC
TGGAATTTGACATCACAGCAACTAGCAATCTGTGGGTGGTGACACCGCAGCACAACATGGGGCTCCAGCT
GAGTGTGGTGACTCGGGATGGACTCCACGTCAACCCCCGTGCGGCGGGCCTGGTGGGCAGAGACGGCCCT
TACGACAAGCAGCCCTTCATGGTGGCCTTCTTCAAGGTGAGCGAGGTCCACGTGCGCACCACCAGGTCAG
CCTCCAGTCGGCGGCGGCAGCAGAGTCGCAACCGGTCCACCCAGTCGCAGGACGTGTCCCGGGGCTCCGG
TTCTTCAGACTACAACGGCAGTGAGTTAAAAACAGCTTGCAAGAAGCATGAGCT
nt 564 - nt 1457 of NM_007556.1
Notes:The Bmp6 (Vgr-1) probe used in this study by Kim et al., 2001 [PMID:11437450] is indicated as that previously described by Lyons et al., 1989 [PMID:2481605] i.e. "An 893-bp SacI-EcoRI fragment from the 5' region of the Vgr-1 cDNA (Lyons et al. 1989 [PMID:2734307] )." Editors note: As restriction analysis of Bmp6/Vgr1 clones in the public databases (eg NM_007556.1) finds an internal SacI cut site, but no 5' EcoRI site, this 5' EcoRI site is presumably linker derived. The fragment shown above extends 5' from the SacI site at nt1457 for 893nt.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Kim RY; Robertson EJ; Solloway MJ, 2001 [PMID:11437450] , Indexed and Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2001.0284] [ PMID:11437450] Kim RY, Robertson EJ, Solloway MJ 2001 Bmp6 and Bmp7 are required for cushion formation and septation in the developing mouse heart. Dev Biol (235):449-66
 [ doi:10.1101/gad.3.11.1657] [ PMID:2481605] Lyons KM, Pelton RW, Hogan BL 1989 Patterns of expression of murine Vgr-1 and BMP-2a RNA suggest that transforming growth factor-beta-like genes coordinately regulate aspects of embryonic developm Genes Dev (3):1657-68
 [ doi:10.1073/pnas.86.12.4554] [ PMID:2734307] Lyons K, Graycar JL, Lee A, Hashmi S, Lindquist PB, Chen EY, Hogan BL, Derynck R 1989 Vgr-1, a mammalian gene related to Xenopus Vg-1, is a member of the transforming growth factor beta gene superfamily. Proc Natl Acad Sci U S A (86):4554-8
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE