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EMAGE:4683

Brca1 breast cancer 1 ( MGI:104537)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4683
Fig5B. from Genes Dev 9(21): 2712-22, Lane TF; Deng C; Elson A; Lyu MS; Kozak CA; Leder P, Expression of Brca1 is associated with terminal differentiation of ectodermally and mesodermally derived tissues in mice. Copyright 1995 [PMID:7590247]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This embryo is described as "de-epithelialized", which was achieved by proteinase treatment.
Expression Pattern Description
Spatial Annotation:
EMAGE:4683Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4683_wholemount_moderate_3D_1.wlz
4683_wholemount_weak_3D_1.wlz
4683_wholemount_notDetected_3D_1.wlz
4683_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4683_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
ear
detected detected
regionalotic vesicle staining is diminished (compared to E9.5).
future forebrain
detected detected
Tissues in the forebrain are distinctly positive.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Brca1 probeB
Entity Detected:Brca1, breast cancer 1 ( MGI:104537)
Notes:The Brca1 probe used in this study by Lane et al., 1995 [PMID:7590247] is described as follows: "Riboprobe templates were generated by linearization of pBSKSII plasmids (Stratagene, La Jolla, CA) that contained a subclone of the 5' 900 bp of Brcal mRNA (probe B)." Editors Note: Probe B is shown schematically in Fig1.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:either: C57BL/6 or Swiss Webster
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Lane TF; Deng C; Elson A; Lyu MS; Kozak CA; Leder P, 1995 [PMID:7590247] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1101/gad.9.21.2712] [ PMID:7590247] Lane TF, Deng C, Elson A, Lyu MS, Kozak CA, Leder P 1995 Expression of Brca1 is associated with terminal differentiation of ectodermally and mesodermally derived tissues in mice. Genes Dev (9):2712-22
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE