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EMAGE:4684

Bsx brain specific homeobox ( MGI:2669849)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4684 EMAGE:4684
Fig2A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00151-0] Gene Expr Patterns 4: 47-51, Cremona M; Colombo E; Andreazzoli M; Cossu G; Broccoli V, Bsx, an evolutionary conserved Brain Specific homeoboX gene expressed in the septum, epiphysis, mammillary bodies and arcuate nucleus. Copyright 2003. [PMID:14678827] Fig2B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S1567-133X(03)00151-0] Gene Expr Patterns 4: 47-51, Cremona M; Colombo E; Andreazzoli M; Cossu G; Broccoli V, Bsx, an evolutionary conserved Brain Specific homeoboX gene expressed in the septum, epiphysis, mammillary bodies and arcuate nucleus. Copyright 2003. [PMID:14678827]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: (A) Lateral view of E9.5 mouse embryo. Arrows indicate positive cells in the anterior brain. (B) Arrows indicates Bsx expressing cells located into the dorsal diencephalons. die - diencephalon; md - midbrain; tel - telencephalon e - eye.
Expression Pattern Description
Spatial Annotation:
EMAGE:4684Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4684_wholemount_possible.wlz
4684_wholemount_notDetected.wlz
4684_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4684_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
diencephalon
detected detected
regionalBsx expression is first detected at E9.5 in few cells located bilaterally in the dorsal diencephalon.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Bsx probeA
Entity Detected:Bsx, brain specific homeobox ( MGI:2669849)
Notes:The Bsx probe used in this study by Cremona et al., 2004 [PMID:14678827] is described as "A 450 bp fragment corresponding to the 3' region of the Bsx coding sequence, cloned in pCRII (Stratagene). Antisense and sense probes were linearizing with XhoI and HindIII and transcribing with T7 and Sp6 RNA polymerase, respectively." Editors Note: the authors do not specify the exact 450bp region this probe covers.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Cremona M; Colombo E; Andreazzoli M; Cossu G; Broccoli V, 2004 [PMID:14678827] , Indexed and Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S1567-133X(03)00151-0] [ PMID:14678827] Cremona M, Colombo E, Andreazzoli M, Cossu G, Broccoli V 2004 Bsx, an evolutionary conserved Brain Specific homeoboX gene expressed in the septum, epiphysis, mammillary bodies and arcuate nucleus. Gene Expr Patterns (4):47-51
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE