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EMAGE:4691

Cartpt CART prepropeptide ( MGI:1351330)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4691
Fig4A. Copyright: This image is from [doi:10.1073/pnas.0602152103] Heanue TA; Pachnis V, Proc Natl Acad Sci U S A 2006 May 2;103(18):6919-24. [PMID:16632597] Copyright 2006 National Academy of Sciences, U.S.A.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: arrowhead indicates Cart expression in a subset of enteric neurons.
Expression Pattern Description
Spatial Annotation:
EMAGE:4691Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4691_wholemount_notDetected_3D_1.wlz
4691_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4691_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
autonomic nervous system
detected detected
regionalCart can be observed within the ENS (enteric nervous system) from E11.5 when ENS precursors are migrating rostrocaudally through the gut.
gut
detected detected
regionalCart can be observed within the ENS (enteric nervous system) from E11.5 when ENS precursors are migrating rostrocaudally through the gut.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Cartpt probeA
Entity Detected:Cartpt, CART prepropeptide ( MGI:1351330)
Sequence:sense strand is shown

>Cartpt probeA
TGGAGAGCTCCCGCCTGCGGCTGCTACCCCTCCTGGGCGCCGCCCTGCTGCTACTGCTACCTTTGCTGGG
TGCCCGTGCCCAGGAGGACGCCGAGCTGCAGCCCCGAGCCCTGGACATCTACTCTGCCGTGGATGATGCG
TCCCACGAGAAGGAGCTGCCAAGGCGGCAACTTCGGGCTCCCGGCGCTATGTTGCAGATCGAAGCGTTGC
AAGAAGTCCTGAAGAAGCTCAAGAGTAAACGCATTCCGATCTACGAGAAGAAGTACGGCCAAGTCCCCAT
GTGTGACGCTGGAGAGCAGTGCGCAGTGAGGAAAGGGGCCAGGATCGGGAAGCTGTGTGACTGTCCCCGA
GGAACTTCCTGCAATTCTTTCCTCTTGAAGTGCTTGTGAAGGGACGACAGCCGCCACCTTCGGTTCCCAT
ATTCCCTCTTTCCCCCAAAGGAGCGCTCCATTATCCCTGGAGCCTGGCTTTAGCAACAATAAAGTTTGCG
TTCCCCTCAGAGAGCGGATGGGCTCTTTCCCTGTTGCTTCAAAATAAAAGATTTGACATCATTGTGTGAA
GGA
nt 51 - nt 613 of NM_013732.6
Notes:The Cartpt (Cart) probe used in this study by Heanue & Pachnis, 2006 [PMID:16632597] was generated from IMAGE clone 466874, digested with EcoRI and transcribed with T3 polymerase. Editors Note: The 5' and 3' sequences of IMAGE:466874 (AI322575 and AI324263 respectively) overlap with the full resulting sequence equivalent to nt51-613 of NM_013732.6. There is no EcoRI site within this sequence, therefore the probe was made against the full insert of IMAGE:466874.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Heanue & Pachnis, 2006 [PMID:16632597] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1073/pnas.0602152103] [ PMID:16632597] Heanue TA, Pachnis V 2006 Expression profiling the developing mammalian enteric nervous system identifies marker and candidate Hirschsprung disease genes. Proc Natl Acad Sci U S A (103):6919-24
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE