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EMAGE:4692

Myh9 myosin, heavy polypeptide 9, non-muscle ( MGI:107717)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4692
Figure 1B. [doi:10.1016/j.ygeno.2003.12.012] Marigo V; Nigro A; Pecci A; Montanaro D; Di Stazio M; Balduini CL; Savoia A, 2004. Correlation between the clinical phenotype of MYH9-related disease and tissue distribution of class II nonmuscle myosin heavy chains. Genomics 83(6): 1125-33.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4692Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4692_wholemount_moderate_3D_1.wlz
4692_wholemount_notDetected_3D_1.wlz
4692_wholemount_possible_3D_1.wlz
4692_wholemount_strong_3D_1.wlz
4692_wholemount_weak_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4692_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
otocyst
detected detected
cardiovascular system
detected detected
regionalExpression in endothelium of vessels in the embryonic head.
central nervous system
not detected not detected
homogeneous
embryo
detected detected
Expression is widely distributed but excluded from the central nervous system.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Myh9 probeA
Entity Detected:Myh9, myosin, heavy polypeptide 9, non-muscle ( MGI:107717)
Sequence:sense strand is shown

>Myh9 probeA
ATAAACACCGACCTGAACCTGGAACGCAGCCACGCACAGAAGAATGAGAATGCGCGACACGAGCTGGAAC
GCCAGAACAAGGAGCTCAAGGCCAAGCTGCAGGAAATGGAGAGTGCTGTCAAGTCCAAATACAAGGCCTC
CATCGCGGCCTTGGAGGCAAAATTGCACAGCTGGAGGAACAGCTGGACAACGAGACCAAGGAGCGCCAGG
CAGCCTCCAAGCAGGTGCGCCGGACGGAGAAGAAGCTGAAGGACGTGCTGCTGCAGGTGGAGGACGAGCG
GAGGAACGCGGAACAGTTCAAGGACCAGGCTGACAAGGCGTCCACCCGCCTGAAGCAGCTTAAACGGCAG
CTAGAGGAGGCTGAAGAGGAGGCCCAGC
Notes:The Myh9 (NMHC II-A) probe used in this study by Marigo et al., 2004 [PMID:15177565] "was obtained by linearization of the EST clone AA575507 (IMAGE) with SalI and transcription with SP6 RNA polymerase". Editors note: AA575507 is the 5' sequence read from the insert of IMAGE:988327
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Marigo V; Nigro A; Pecci A; Montanaro D; Di Stazio M; Balduini CL; Savoia A, 2004 [PMID:15177565] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.ygeno.2003.12.012] [ PMID:15177565] Marigo V, Nigro A, Pecci A, Montanaro D, Di Stazio M, Balduini CL, Savoia A 2004 Correlation between the clinical phenotype of MYH9-related disease and tissue distribution of class II nonmuscle myosin heavy chains. Genomics (83):1125-33
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE