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EMAGE:4695

Myh6 myosin, heavy polypeptide 6, cardiac muscle, alpha ( MGI:97255)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4695
Fig6_E9.5_Myhca. Copyright: This image is from [doi:10.1002/dvdy.20136] [PMID:12761855] Porter GA, Makuck RF, Rivkees SA, "Intracellular calcium plays an essential role in cardiac development" Dev Dyn 2004 227(2): 280-290, and is displayed with the permission of Wiley-Liss Inc. who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4695Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4695_wholemount_possible_3D_1.wlz
4695_wholemount_notDetected_3D_1.wlz
4695_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4695_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
regionalExpressed in differentiated cardiac myocytes
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Myh6 probeA
Entity Detected:Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha ( MGI:97255)
Sequence:sense strand is shown

>Myh6 probeA
GCCACTGATAGTGCCTTTGATGTGCTGAGCTTCACGGCAGAGGAGAAGGCTGGTGTCTACAAGCTGACAG
GGGCCATCATGCACTACGGAAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGGCAGAGCCTGATGG
CACAGAAGATGCTGACAAATCAGCCTACCTCATGGGGCTGAACTCAGCTGACCTGCTCAAGGGCCTGTGT
CACCCTCGGGTGAAGGTGGGGAACGAGTATGTCACCAAGGGGCAGAGTGTACAGCAAGTGTACTATTCCA
TCGGGGCACTGGCCAAGTCAGTGTACGAGAAGATGTTCAACTGGATGGTGACACGCATCAACGCAACCCT
GGAGACCAAGCAGCCGCGCCAGTACTTCATAGGTGTCCTGGACATTGCCGGCTTTGAGATCTTCGATTTC
AACAGCTTTGAGCAGCTGTGCATCAACTTCACCAATGAGAAGCTGCAGCAGTTCTTCAACCACCACATGT
TCGTGCTGGAGCAGGAGGAGTACAAGAAGGA
nt 1096 - nt 1616 of NM_010856.2
Notes:The Myh6 (Myhca) probe used in this study by Porter et al., 2003 [PMID:12761855] was ontained by RT-PCR using the following primers: 5'-GCCACTGATAGTGCCTTTGATGTG-3', 5'-TCCTTCTTGTACTCCTCCTGCTCC-3'
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6J
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Porter GA Jr; Makuck RF; Rivkees SA, 2003 [PMID:12761855] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.10307] [ PMID:12761855] Porter GA, Makuck RF, Rivkees SA 2003 Intracellular calcium plays an essential role in cardiac development. Dev Dyn (227):280-90
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE