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EMAGE:4697

Myl2 myosin, light polypeptide 2, regulatory, cardiac, slow ( MGI:97272)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4697
Fig6D. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.9748] [PMID:11884037] Dev Biol 223( 1): 169-180. Christine M. Liberatore, Robin D. Searcy-Schrick and Katherine E. Yutzey; Ventricular Expression of tbx5 Inhibits Normal Heart Chamber Development. Copyright 2000.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4697Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4697_wholemount_notDetected_3D_1.wlz
4697_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4697_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Myl2 probeA
Entity Detected:Myl2, myosin, light polypeptide 2, regulatory, cardiac, slow ( MGI:97272)
Notes:The Myl2 (MLC2V) probe used in this study by Liberatore et al., 2000 [PMID:10864469] is indicated as that originally described by Molkentin et al., 1997 [PMID:9136933] where a plasmid for MLC2V was cut and "antisense transcripts were generated with DdeI and T7".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:FVBN
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Liberatore CM; Searcy-Schrick RD; Yutzey KE, 2000 [PMID:10864469] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2000.9748] [ PMID:10864469] Liberatore CM, Searcy-Schrick RD, Yutzey KE 2000 Ventricular expression of tbx5 inhibits normal heart chamber development. Dev Biol (223):169-80
 [ doi:10.1101/gad.11.8.1061] [ PMID:9136933] Molkentin JD, Lin Q, Duncan SA, Olson EN 1997 Requirement of the transcription factor GATA4 for heart tube formation and ventral morphogenesis. Genes Dev (11):1061-72
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE