Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:4702

Nek2 NIMA (never in mitosis gene a)-related expressed kinase 2 ( MGI:109359)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4702
Figure 6C. [doi:10.1006/excr.1997.3788] Exp Cell Res 237(2):264-74 (1997). Tanaka K; Parvinen M; Nigg EA, "The in vivo expression pattern of mouse Nek2, a NIMA-related kinase, indicates a role in both mitosis and meiosis." Exp Cell Res 1997 Dec 15;237(2):264-74

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4702Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4702_wholemount_moderate_3D_1.wlz
4702_wholemount_possible_3D_1.wlz
4702_wholemount_notDetected_3D_1.wlz
4702_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4702_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
strong strong
midbrain
strong strong
Strong expression in mesencephalon
branchial arch
not detected not detected
homogeneous
future spinal cord
detected detected
regionalExpression limited to the anterior.
hindbrain
detected detected
gradedStrong expression in metencephalon, with signal decreasing towards the myelencephalon and anterior neural tube
otocyst
possible possible
Some staining of the auditory vesicles and olfactory pit was observed with both antisense and sense probes, suggesting that this nonspecific staining may be due to the poor accessibility of these regions to washing buffer.
olfactory pit
possible possible
Some staining of the auditory vesicles and olfactory pit was observed with both antisense and sense probes, suggesting that this nonspecific staining may be due to the poor accessibility of these regions to washing buffer.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Nek2 probeA
Entity Detected:Nek2, NIMA (never in mitosis gene a)-related expressed kinase 2 ( MGI:109359)
Notes:The Nek2 in situ hybridisation probe used in this study by Tanaka et al., 1997 [PMID:9434622] was transcribed from a "Nek2 cDNA fragment". Editors note: This may be the same Nek2 cDNA fragment that was used by the authors to generate a probe for RNAse protection assays (nt 700-1409 of the sequence shown in Fig 1 (AF007247.1)), although the authors do not explicitly state this.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CBA * C57BL/6
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Tanaka K; Parvinen M; Nigg EA, 1997 [PMID:9434622] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/excr.1997.3788] [ PMID:9434622] Tanaka K, Parvinen M, Nigg EA 1997 The in vivo expression pattern of mouse Nek2, a NIMA-related kinase, indicates a role in both mitosis and meiosis. Exp Cell Res (237):264-74
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE