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EMAGE:4704

Nes nestin ( MGI:101784)
TS17 (10.5 dpc)
in situ reporter

Data Images
EMAGE:4704
Figure 3A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.mod.2003.11.004] Mech Dev 121: 3-14, Delacour A; Nepote V; Trumpp A; Herrera PL, Nestin expression in pancreatic exocrine cell lineages. Copyright 2004. [PMID:14706695]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4704Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4704_wholemount_moderate_3D_1.wlz
4704_wholemount_notDetected_3D_1.wlz
4704_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4704_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
central nervous system
strong strong
regionalExpression in encephalon and neural tube
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Nes, nestin ( MGI:101784)
Notes:LacZ expression, driven by Nes control elements was detected in this study by Delacour et al., 2004 [PMID:14706695] as follows: Two different nestin-Cre mice (Tronche et al., 1999 [PMID:10471508] Jackson Lab strain: B6.Cg (SJL)-TgN(NesCre)1Kln; stock #003771) and (Trumpp et al., 1999 [PMID:10601039] ), both of which bear a Cre recombinase gene under the control of the rat nestin 5' Flanking (5.8-kb) and 2nd intron (1.8-kb) sequences, as described (Zimmerman et al., 1994 [PMID:8292356] ) were crossed with the ROSA26R ubiquitous reporter mice (R26R) (Soriano, 1999 [PMID:9916792] Jackson Lab strain: B6.129S4-Gtrosa26tm1Sor; stock #003474). Doubly transgenic animals were treated with X-Gal for anti-beta-galactosidase staining. The authors state that both transgenic strains of mice, encoding Cre under the control of rat nestin cis-regulatory sequences yielded the same expression pattern, but do not state which strain was used in this particular assay.
Labelled with:LacZ
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:nestin-Cre/R26R transgenic
Preparation:wholemount
Procedures
General Information
Authors:Delacour A; Nepote V; Trumpp A; Herrera PL, 2004 [PMID:14706695] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.mod.2003.11.004] [ PMID:14706695] Delacour A, Nepote V, Trumpp A, Herrera PL 2004 Nestin expression in pancreatic exocrine cell lineages. Mech Dev (121):3-14
 [ doi:10.1101/gad.13.23.3136] [ PMID:10601039] Trumpp A, Depew MJ, Rubenstein JL, Bishop JM, Martin GR 1999 Cre-mediated gene inactivation demonstrates that FGF8 is required for cell survival and patterning of the first branchial arch. Genes Dev (13):3136-48
 [ doi:10.1038/12703] [ PMID:10471508] Tronche F, Kellendonk C, Kretz O, Gass P, Anlag K, Orban PC, Bock R, Klein R, Schütz G 1999 Disruption of the glucocorticoid receptor gene in the nervous system results in reduced anxiety. Nat Genet (23):99-103
 [ doi:10.1016/0896-6273(94)90148-1] [ PMID:8292356] Zimmerman L, Parr B, Lendahl U, Cunningham M, McKay R, Gavin B, Mann J, Vassileva G, McMahon A 1994 Independent regulatory elements in the nestin gene direct transgene expression to neural stem cells or muscle precursors. Neuron (12):11-24
 [ doi:10.1038/5007] [ PMID:9916792] Soriano P 1999 Generalized lacZ expression with the ROSA26 Cre reporter strain. Nat Genet (21):70-1
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE