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EMAGE:4712

Nhlh1 nescient helix loop helix 1 ( MGI:98481)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4712
Figure 5D. Fig1A. Copyright: Reprinted with permission from Cell Press from [doi:10.1016/S0896-6273(00)80989-7] Neuron 20: 483-94 Neuron 133: 142-153, Fode C; Gradwohl G; Morin X; Dierich A; LeMeur M; Goridis C ; Guillemot F, "The bHLH protein NEUROGENIN 2 is a determination factor for epibranchial placode-derived sensory neurons." Copyright 1998. [PMID:9539123]

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Abbreviations: ov, otic vesicle; VII, geniculate placode ganglion; IX, petrosal placode/ganglion; X, nodose placode/ganglion.
Expression Pattern Description
Spatial Annotation:
EMAGE:4712Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4712_wholemount_moderate_3D_1.wlz
4712_wholemount_notDetected_3D_1.wlz
4712_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4712_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
glossopharyngeal ix preganglion
detected detected
Expression in petrosal preganglion.
vagus x preganglion
detected detected
Expression in nodose preganglion.
facio-acoustic vii-viii preganglion complex
detected detected
regionalExpressed in geniculate (VII) preganglion.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Nhlh1 probeA
Entity Detected:Nhlh1, nescient helix loop helix 1 ( MGI:98481)
Notes:The Nhlh1 (NSCL1/Nscll) probe used in this study by Fode et al., 1998 [PMID:9539123] is indicated as that used by Begley et al, 1992 [PMID:1729708] . Editors note: Begley et al describe two probes which were transcribed from "two different NSCL cDNA clones in pBluescript by using either T7 or T3 RNA polymerase (BRL) according to the manufacturer's instructions. One cDNA clone (1.5 kilobases (kb)) extended to the EcoRI site in the 3' untranslated region (UTR) and included the entire NSCL coding region and 5' UTR. A second cDNA clone encompassed 600 base pairs (bp) of 3' UTR sequence beginning at the EcoRI site. Both probes gave identical results". Fode et al, do not specify which of these two probes was used.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Fode C; Gradwohl G; Morin X; Dierich A; LeMeur M; Goridis C ; Guillemot F, 1998 [PMID:9539123] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0896-6273(00)80989-7] [ PMID:9539123] Fode C, Gradwohl G, Morin X, Dierich A, LeMeur M, Goridis C, Guillemot F 1998 The bHLH protein NEUROGENIN 2 is a determination factor for epibranchial placode-derived sensory neurons. Neuron (20):483-94
 [ doi:10.1073/pnas.89.1.38] [ PMID:1729708] Begley CG, Lipkowitz S, Gobel V, Mahon KA, Bertness V, Green AR, Gough NM, Kirsch IR 1992 Molecular characterization of NSCL, a gene encoding a helix-loop-helix protein expressed in the developing nervous system. Proc Natl Acad Sci U S A (89):38-42
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE