EMAGE mouse gene expression spatial database

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EMAGE:4713

Nhlh2 nescient helix loop helix 2 ( MGI:97324)
TS19 (11.5 dpc)
in situ hybridisation

Data Images


Fig4B of [doi:10.1038/ng0497-397] Good DJ; Porter FD; Mahon KA; Parlow AF; Westphal H; Kirsch IR, "Hypogonadism and obesity in mice with a targeted deletion of the Nhlh2 gene." Nat Genet 1997 Apr;15(4): 397-401. [PMID:9090387] © Nature Publishing Group. Reproduced with permission from Nat Genet.	Fig4C of [doi:10.1038/ng0497-397] Good DJ; Porter FD; Mahon KA; Parlow AF; Westphal H; Kirsch IR, "Hypogonadism and obesity in mice with a targeted deletion of the Nhlh2 gene." Nat Genet 1997 Apr;15(4): 397-401. [PMID:9090387] © Nature Publishing Group. Reproduced with permission from Nat Genet.	Fig4D of [doi:10.1038/ng0497-397] Good DJ; Porter FD; Mahon KA; Parlow AF; Westphal H; Kirsch IR, "Hypogonadism and obesity in mice with a targeted deletion of the Nhlh2 gene." Nat Genet 1997 Apr;15(4): 397-401. [PMID:9090387] © Nature Publishing Group. Reproduced with permission from Nat Genet.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: The planes of sections C and D are indicated in B. LV, lateral ventricles; III, third ventricle; T, telencephalon; H,hypothalamus; LH, lateral hypothalamus; VMH, ventromedial hypothalamus.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4713_wholemount__moderate.wlz

4713_wholemount__weak.wlz

4713_wholemount__possible.wlz

4713_wholemount__notDetected.wlz

4713_wholemount__strong.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4713_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

detected

Expression is in the hypothalamus, regionalised to the differentiating zones of the ventromedial and lateral hypothalamus which overlap the eventual location of the arcuate, ventromedial and the lateral hypothalamus.

telencephalon

detected

3rd ventricle

detected

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Nhlh2 probeA
Entity Detected:	Nhlh2, nescient helix loop helix 2 ( MGI:97324)
Notes:	The Nhlh2 (NSCL-2) probe used in this study by Good et al., 1997 [PMID:9090387] was generated from a "1.9-kB cDNA insert (Gobel et al., 1992 [PMID:1633105] ". Editors Note: Gobel et al. cloned a two overlapping cDNA fragments of mouse NSCL-2 (with a predicted overall cDNA length 2.2kb+) and performed in situ hybridisations, however the region of the cDNA that the in situ probe covered (or its length) is not reported.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	11.5 dpc
Theiler Stage:	TS19
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

General Information

Authors:	Good DJ; Porter FD; Mahon KA; Parlow AF; Westphal H; Kirsch IR, 1997 [PMID:9090387] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1038/ng0497-397] [ PMID:9090387] Good DJ, Porter FD, Mahon KA, Parlow AF, Westphal H, Kirsch IR 1997 Hypogonadism and obesity in mice with a targeted deletion of the Nhlh2 gene. Nat Genet (15):397-401
	[ PMID:1633105] G�bel V, Lipkowitz S, Kozak CA, Kirsch IR 1992 NSCL-2: a basic domain helix-loop-helix gene expressed in early neurogenesis. Cell Growth Differ (3):143-8
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source