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EMAGE:4715

Nkd2 naked cuticle 2 homolog (Drosophila) ( MGI:1919543)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4715 EMAGE:4715
Figure 4B. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0238] Wharton KA Jr; Zimmermann G; Rousset R; Scott MP, "Vertebrate proteins related to Drosophila Naked Cuticle bind Dishevelled and antagonize Wnt signaling." Dev Biol 2001 Jun 1;234(1):93-106. Copyright 2001 [PMID:11356022] Figure 4D. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0238] Wharton KA Jr; Zimmermann G; Rousset R; Scott MP, "Vertebrate proteins related to Drosophila Naked Cuticle bind Dishevelled and antagonize Wnt signaling." Dev Biol 2001 Jun 1;234(1):93-106. Copyright 2001 [PMID:11356022]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: arrowheads designate an absence of dorsal CNS staining. D is a close-up of the limb region in B.
Expression Pattern Description
Spatial Annotation:
EMAGE:4715Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4715_wholemount_moderate.wlz
4715_wholemount_weak.wlz
4715_wholemount_possible.wlz
4715_wholemount_notDetected.wlz
4715_wholemount_strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4715_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
neural tube
not detected not detected
homogeneousNot expressed in dorsal neural tube.
trunk somite
detected detected
regionalTranscripts accumulate at the anterior and posterior of each somite boundary and are distributed in a gradient emanating from somites.
forelimb bud
detected detected
homogeneous
branchial arch
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Nkd2 probeA
Entity Detected:Nkd2, naked cuticle 2 homolog (Drosophila) ( MGI:1919543)
Sequence:sense strand is shown

>Nkd2 probeA
GAGCAGGCCAGGCAAGAACATCATGGCAGGGCCACACACATTCCAAGCAGGTCCCGATCACAAGAGTCGG
ATGCCCACGCTATACACCACCGCAGGTCTCAAGTCCTGGCTGAGCATGTCATACCAGCTAATGAGCCTGC
CACCCGGGCCCTGGCTGCACAGCCCCGGATCAAGGGGCAGGAGAAGCAGTTCCTCAGGTCTCCTAAGGGT
CCAGGAAAACCTCTTGGGACACCAGGCAGTGGCAAGCCAGGGAAAGCTCTCAGCTATTGCCTGCAGGCCG
TGCCATTGCCCCAGAGTGCTCAGGATGGCCACCACCTTCCTCAGCCCCCACCACAGCCTCCACCGCAGCC
CTATGGTCACAAGCGGTACCGGCAGAAAGCCAGAGAAGGCCACTCACCACTTAAGGGGCATGGCCAGCCT
ACCATGGTGGAGCATGAAGTAGTACGGGACCTGCCTCCCATGCTGGGGCCTGAGGGCTATGTGATGCCTG
TGGTCC
nt 974 - nt 1469 of NM_028186.1
Notes:The Nkd2 probe used in this study by Wharton et al., 2001 [PMID:11356022] corresponds to "amino acids 273 to 438 in mNkd2".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Wharton KA Jr; Zimmermann G; Rousset R; Scott MP, 2001 [PMID:11356022] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2001.0238] [ PMID:11356022] Wharton KA, Zimmermann G, Rousset R, Scott MP 2001 Vertebrate proteins related to Drosophila Naked Cuticle bind Dishevelled and antagonize Wnt signaling. Dev Biol (234):93-106
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE