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EMAGE:4718

Nme1 non-metastatic cells 1, protein (NM23A) expressed in ( MGI:97355)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4718
Figure 2C. [doi:10.1007/s00441-005-0036-9] Amrein L; Barraud P; Daniel JY; Perel Y; Landry M, "Expression patterns of nm23 genes during mouse organogenesis." Cell Tissue Res 2005 Dec;322(3):365-78.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4718Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4718_wholemount_moderate_3D_1.wlz
4718_wholemount_weak_3D_1.wlz
4718_wholemount_notDetected_3D_1.wlz
4718_wholemount_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4718_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
neural tube
detected detected
dorsal root ganglion
detected detected
diencephalon
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Nme1 probeA
Entity Detected:Nme1, non-metastatic cells 1, protein (NM23A) expressed in ( MGI:97355)
Notes:The Nme1 (nm23-M1) probe template used in this study by Amrein et al., 2005 [PMID:16082520] is defined as follows: "A 163-bp nm23-M1 specific probe containing in the 3' untranslated region of the nm23-M1 gene was generated by polymerase chain reaction and gel purification and subcloned into pBluescript II SK-vector (Stratagene, La Jolla, Calif., USA)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Amrein L; Barraud P; Daniel JY; Perel Y; Landry M, 2005 [PMID:16082520] . Indexed and spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1007/s00441-005-0036-9] [ PMID:16082520] Amrein L, Barraud P, Daniel JY, P�rel Y, Landry M 2005 Expression patterns of nm23 genes during mouse organogenesis. Cell Tissue Res (322):365-78
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE