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EMAGE:4725

Casp8 caspase 8 ( MGI:1261423)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4725
Fig1C. from [doi:10.1016/S1074-7613(00)80609-3] Immunity 9(2): 267-76, Varfolomeev EE; Schuchmann M; Luria V; Chiannilkulchai N; Beckmann JS; Mett IL; Rebrikov D; Brodianski VM; Kemper OC; Kollet O; Lapidot T; Soffer D; Sobe T; Avraham KB; Goncharov T; Holtmann H; Lonai P; Wallach D, Targeted disruption of the mouse Caspase 8 gene ablates cell death induction by the TNF receptors, Fas/Apo1, and DR3 and is lethal prenatally. Copyright 1998 [PMID:9729047]

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: ba - branchial arches; flb - forelimb bud; hlb - hindlimb bud; ht - heart. Arrow - area of the mid-hind brain junction.
Expression Pattern Description
Spatial Annotation:
EMAGE:4725Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4725_wholemount_moderate.wlz
4725_wholemount_weak.wlz
4725_wholemount_strong.wlz
4725_wholemount_possible.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4725_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
There was widespread distribution of Casp8 transcripts.
heart
strong strong
Elevated levels of expression in the heart (especially the primitive ventricle)
primitive ventricle
strong strong
Elevated signals could be detected in the heart (especially in the primitive ventricle).
forelimb bud ectoderm
strong strong
Elevated levels of expression in the ectoderm of the fore- and hind-limb buds
hindlimb bud ectoderm
strong strong
Elevated levels of expression in the ectoderm of the fore- and hind-limb buds
branchial arch
strong strong
Elevated levels of expression in the branchial arches
hindbrain
strong strong
regionalElevated signals could be detected in the area of the mid-hind brain junction.
midbrain
strong strong
regionalElevated signals could be detected in the area of the mid-hind brain junction.
rest of foregut dorsal mesentery
strong strong
Elevated signals could be detected in the region of the dorsal mesentery.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Casp8 probeA
Entity Detected:Casp8, caspase 8 ( MGI:1261423)
Notes:The Casp8 probe used in this study by Varfolomeev et al., 1998 [PMID:9729047] is described as "single-strand digoxigenin-UTP-labeled mouse Casp8 antisense RNA probe". Editors Note: the sequence/region covered by the probe is not reported by the authors.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Varfolomeev EE; Schuchmann M; Luria V; Chiannilkulchai N; Beckmann JS; Mett IL; Rebrikov D; Brodianski VM; Kemper OC; Kollet O; Lapidot T; Soffer D; Sobe T; Avraham KB; Goncharov T; Holtmann H; Lonai P; Wallach D, 1998 [PMID:9729047] , Indexed and Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S1074-7613(00)80609-3] [ PMID:9729047] Varfolomeev EE, Schuchmann M, Luria V, Chiannilkulchai N, Beckmann JS, Mett IL, Rebrikov D, Brodianski VM, Kemper OC, Kollet O, Lapidot T, Soffer D, Sobe T, Avraham KB, Goncharov T, Holtmann H, Lonai P, Wallach D 1998 Targeted disruption of the mouse Caspase 8 gene ablates cell death induction by the TNF receptors, Fas/Apo1, and DR3 and is lethal prenatally. Immunity (9):267-76
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE